118 Iiwestiyaiious on the Life-History 



with a large amount of soluble proteicl in the muscle. As the season 

 advances, and the fish has been up the river for some time, the amount 

 of soluble proteid in the muscle shows a very decided diminution. The 

 muscle is relatively poor in soluble proteid. Danilewsky (13) has shown 

 that in warm-blooded animals the muscles that have least work to do 

 are richest in globulin, the globulin in active muscles being probably 

 used up in doing their work. The same may be held in the case of the 

 fish. It passes up the river with a large store of soluble proteid in its 

 muscle. As time advances and its muscles are called upon to supply 

 energy, not only does the total amount of its muscle greatly diminish, 

 but there is a marked diminution in the proportional amount of soluble 

 proteid in the muscle. This difference is considerable, amounting (as 

 shown in the Tables) to 0-918 grammes per cent, of soluble proteid a 

 percentage loss of 27 per cent. A comparison of this with the results 

 obtained by Dr. Dunlop (p. 124) indicates that probably the proteid 

 lost from the muscle is derived from these soluble proteids. 



There are two fish of special interest. The fish from the Dee (No. 72), 

 the last in the first Table, was noted on arrival to be specially well 

 nourished ; and on estimating the total soluble proteids they were found 

 to be in very large amount. In the case of a kelt which was examined 

 (an ill-nourished, lean fish which had not been down to the sea to feed), 

 the proportion of soluble proteids was unusually low. 



CONCLUSIONS. 



From the foregoing the following conclusions may be deduced : 



(1) The salmon muscle contains three soluble proteids Musculin or 

 Paramyosinogen, Myosinogen, and Myoglobulin. 



(2) That soluble Myosin-fibrin is rapidly formed from Pai-amyosinogen 

 at ordinary temperatures. 



3) That these proteids are of the nature of globulins. 



4) That salmon muscle contains no albumin. 



5) That salmon muscle contains no proteoses and no peptone. 



6) That salmon muscle contains an insoluble albuminous body 

 which contains phosphorus Myostromin, which is probably a globulin 

 combined with a true or a pseudo-nuclein. 



(7) That the curd of salmon muscle is a proteid body. 



(8) That there is a marked diminution in the percentage of soluble 

 proteids in salmon muscle in fish which have been in the river for some 

 time. 



(9) That the muscle of the kelt is very poor in soluble proteid. 



REFERENCES TO LITERATURE. 



1. Tiegel Pfliigers Archiv. Vol. XXXII., s. 278. 



2. Buchart Arch, fiir exper. Path, und Phar. Vol. XVI., .s. 322. 



3. Salvioli Du Bois Reymonds Archiv. fiir Physiol. Suppliment 

 Band. 1879, s. 273. 



4. Von Fiirth Arch, fur exper. Path, und f'har. Bd. 36. 

 1895, s. 235. 



5. Halliburton Journal of Physiology. Vol. Vlll. 1887, p. 188. 



6. Fischel Zeitschr. fur physiol. Chemie. Vol. X., s. 14. Muira 

 Virchow's Archiv. Vol. 01., s. 316. 



7. ~W bitfield Journal of Physiology. Vol. XVI., p. 487. 



8. Kara jew Wratsch. 1895, No. 39, s. 1083. 



9. Peckelharinsr Zeitschr. fUr phys. Chemie. Bd. XXII.. Heft 3, 

 8. 245. 



