of the Salmon in Fresh Water. 147 



Magnesium Phosphate = -037 grin. 



= -259 grin. Lecithin. 



After extraction of the fats with ether, lecithin is left in closer 

 combination with the proteids. 



2. Presence of Phosphorus in Proteid. 



After extraction with hot alcohol and ether, and drying at 100" 

 C., 1-134 grm. of the proteid were used for determination of 

 phosphorus. 



Magnesium Phosphate - -031 grm. 



= '0086 grm. Phosphorus. 

 = '74 per cent, of Phosphorus. 

 After removal of the lecithin the proteid contains a further amount cf 



3. Reactions of Proteid of Ovary. The proteid was found to be 

 insoluble in water ; soluble in dilute KHO, Na 2 C0 3 , and in neutral 

 salts. On the addition of an acid ic is precipitated, and is soluble only 

 in a considerable excess of acetic acid, but in a small excess of mineral 

 acids, e.g., -2 per cent. HC1. In a weak solution of neutral salts it is 

 not precipitated on boiling; but in a saturated solution of NaCl, in 

 which it is soluble, a copious precipitate is thrown down on heating. It 

 is precipitated by a half-saturated solution of (NH 4 ) 2 SO 4 . The 

 filtrate gives a cloud on boiling and the proteid reactions so a trace of 

 an albumin is present. A stream of CO., gives no precipitate. After 

 being precipitated for some time the proteid loses its solubility in 

 neutral salts. 



4- Does the Proteid contain a Nuclein or Psuedo- Nuclein ? 2'26 grm. 

 were digested for 20 hours in artificial gastric juice. The residue on 

 drying weighed 0'109 grm. 



A large quantity of the proteid was dissolved in -2 per cent. HG1 and 

 liquor pepticus added. On digesting a copious precipitate was thrown 

 down. This precipitate was washed with hot alcohol and ether, and the 

 phosphorus determined in '626 grm. ; it contained 0'0237 grins. P., or 

 3-7 per cent. 



A considerable quantity of the moist proteid was boiled in 5 per cent. 

 H 2 SO 4 for several hours. A brownish solution resulted, with a slight brown 

 precipitate. This was filtered off. The filtrate was treated with baryta 

 water to neutralisation; the baryta was separated by a stream of CO., ; 

 the fluid was then filtered and ammonia added. No precipitate formed. 

 Ag N0 3 was then added, and no precipitate resulted. Hence nuclein 

 bases are absent. The brownish solution, when rendered alkaline and 

 boiled with Fehling's solution caused no reduction. 



5. The, Proteid contains Iron. (Note by E. D. W. Greiy, M.B., C.M.) 

 In the earlier analyses of the ovarian proteid the iron was not 

 determined. 



In an elaborate investigation Walter (Zeitschrift f iir physiol. Chemie, 

 Bd. XV., 1891, p. 477) showed that in the ovary of the carp both the 

 proteid and the nuclein derived from it contained iron. 



I have also determined the quantity of iron present in the pure 

 ovarian proteid, and also in the nuclein obtained from it by artificial 

 digestion. 



The proteid was obtained in the following manner : The fresh ovary 

 was passed through a mincer, and then strained through muslin. It was 

 then repeatedly extracted with ether, and about four times its volume 

 of salt solution (strong) added. This was then poured into a large 

 quantity of distilled water and repeatedly washed by decantation. It 



