INTRODUCTION. 3 



3. The advanced student who may be desirous of 

 working through the following course of instruction pri- 

 vately without the resources of a laboratory requires, in 

 addition to the foregoing apparatus and reagents, a magni- 

 fying power of about 800 or 1000 diam. ; a stage and an 

 eye-piece micrometer ( 59, 60) ; a freezing microtome 

 ( 3 02 ) > a camera lucida ( 58, a or b} ; a lamp with a chim- 

 ney of pale blue glass for night work ( 51) ; a warm stage 

 ( 261); a small and a large injection syringe (Fig. 60) , 

 and perhaps a pressure bottle injection apparatus (Fig. 61) ; 

 a turn-table (Fig. 63). A polarising apparatus may be 

 added, but other accessories are of very little use. Various 

 other reagents are required, but these need not be particu- 

 larised here. 



PREPARATION OF TISSUES PREVIOUS TO THEIR 

 EXAMINATION.* 



4. The following fluids require to be prepared : 



I. f- per cent Salt Solution. Dissolve 7*5 grammes 

 of dried ordinary NaCl in loooCC distilled water. 



II. One per cent Chromic Acid Solution. Dissolve 

 10 grammes chromic acid in loooCC water. This can be 

 readily diluted when required. 



III. Chromic Acid and Spirit Solution. Dissolve i 

 gramme chromic acid in 2oCC water, and slowly add it to 

 iSoCC rectified spirit. 



IV. Chromic and Bichromate Solution. Dissolve i 

 gramme chromic acid, 2 grammes potassium bichromate 

 in i20oCC water. 



V. Chromic and Nitric Fluid. Chromic acid i gramme, 

 water 2ooCC, then add 2CC nitric acid. 



VI. Miller's Fluid. Dissolve 25 grammes potass, 

 bichrom. and 10 grammes sodium sulphate in loooCC 

 water. 



VII. One per cent Bichromate Solution. Dissolve 10 

 grammes potass, bichromate in loooCC water. 



* This section is designed to assist the advanced student when he 

 undertakes histological work in private. 



