INTRODUCTION. 5 



of a rabbit prepared as follows : Divide the kidneys longi- 

 tudinally ; place them for a fortnight in a i per cent solu- 

 tion of chromic acid, then in rectified spirit till required. 



i o. Cut half of the liver into small pieces, and place them 

 in Miiller's fluid. Change the fluid at the end of eighteen 

 hours ; and at the end of four weeks transfer them to 

 rectified spirit till required. 



1 1. Place the spleen, uterus, some thin muscles from 

 the limbs or abdomen, in J per cent chromic acid. Change 

 the fluid at the end of eighteen hours ; and at the end of a 

 month transfer them to rectified spirit. 



12. Through several points in the tunica albuginea of 

 testis of cat, inject a i per cent solution of osmic acid, 

 and then place it in rectified spirit ; and finally a day or 

 two before making sections in absolute alcohol. 



1 2 A. Place the ovaries of cat or dog in Miiller's fluid. 

 Change the fluid after the first day, and after three weeks 

 transfer to a mixture of equal parts of absolute alcohol and 

 water for. thirty-six or forty-eight hours, then in- absolute 

 alcohol for a day, after which they will be ready for section. 



13. Remove both eyes. Divide them transversely behind 

 the crystalline lens. Remove the vitreous. Place the pos- 

 terior halves in the chromic and spirit (Solution 3). Change 

 the fluid at the end of eighteen hours. Transfer to recti- 

 fied spirit at the end of ten days. Place the crystalline 

 lens in chromic acid and spirit for two weeks, and then in 

 rectified spirit. Place the cornea in J- per cent chromic 

 acid for a month, and then in rectified spirit. 



14. Cautiously open the cranial and spinal cavities. 

 Remove brain and spinal cord, and strip off arachnoid. 

 Partially divide spinal cord into pieces half an inch long. 

 Partially divide the brain transversely into a number of 

 pieces. Place them in a cool place in rectified spirit for 

 eighteen hours. Transfer the spinal cord to per cent 

 chromic acid for six or seven weeks. Change the fluid at 

 the end of eighteen hours. Prepare the sciatic nerve in 

 the same manner. Place the brain in chromic and bi- 

 chromate fluid. Change at the end of eighteen hours, and 

 then once a week, until the brain is hard and tough. If it 



