HISTOLOGICAL DEMONSTRATIONS. 75 



fascia from the leg, or of sub-peritoneal tissue from the lum- 

 bar region of a frog recently killed. A small portion of the 

 tissue is excised with scissors, and gently spread it out on a 

 slide in a drop of aqueous humour ( 265). Observe the 

 stationary and amoeboid connective tissue corpuscles. The 

 latter, often termed the wandering cells of connective tissue, 

 appear to be identical with white blood corpuscles. The 

 former are the true connective tissue corpuscles. Endeavour 

 to see the locomotion of the wandering cells. The changes 

 of shape, but without locomotion, of the proper connective 

 tissue corpuscles may perhaps also be seen. 



102. Connective tissue corpuscles may also be advan- 

 tageously studied in the loose subcutaneous tissue of the 

 groin of a young rabbit, cat, or guinea-pig, recently killed. 

 The tissue should be gently spread out on the dry slide, as 

 in 100, and magenta added before the tissue becomes 

 dry. This dye reveals the corpuscles with great readiness. 

 The connective tissue corpuscles proper are usually flattened. 

 When seen in profile they are often fusiform. Some are 

 continuous with elastic fibres, others have no distinct pro- 

 cesses. 



103. (H.) Examine the preparation of omentum 

 already made (870), and observe the connective tissue cor- 

 puscles amidst the fibres, and the epithelium here and there 

 clasping the bundles of fibres. Amoeboid cells (no longer 

 moving, of course) may sometimes be seen singly or in groups 

 here and there on the surface of the bundles. 



104. Tendon. Sections of fresh tendon may be readily 

 made with the freezing microtome ( 302) ; or the tissue 

 may be hardened in \ per cent chromic acid, or hardened 

 by drying, and then cut ; the tendon being either simply 

 held in the hand or imbedded in paraffin and cut in the 

 microtome ( 303). 



Dry a tendon of an animal such as a sheep or a rabbit, 

 until it is just dense enough to be readily sliced. Make an 

 excessively thin transverse section with a scalpel or a razor. 

 The former is preferable, as the tendon is often over-dried, 

 and in that case apt to spoil a blade so thin as that of a 

 razor. The knife should be wetted with a spirit, for water 



