H4 PRACTICAL HISTOLOGY. 



b. The cells of the peptic glands are remarkably well seen in a 

 stomach hardened in osmic acid. A portion of the mucous membrane 

 is separated from the muscular coat of the stomach of a cat just killed. 

 The mucus is washed away with a stream of salt solution. One or 

 two pieces about an eighth of an inch square are placed in \ per cent 

 osmic acid for twenty-four hours. It may then be readily cut in the 

 freezing microtome, and the sections mounted in glycerine or Farrants' 

 solution. The nuclei of the cells may be well brought into view by 

 slight staining with logwood. 



c. Sections of the fresh stomach may be readily made in the freezing 

 microtome, and stained with magenta or a watery solution of anilin blue. 



189. Structure. V. S. chromic acid stomach of cat; 

 mount in glycerine. 



(L.) The relations of the mucous, submucous, and 

 muscular coats. 



(H.) The mucous coat. The peptic follicles cut vertically. 

 The columnar epithelium at their open ends, and the 

 epithelial cells of irregular shape throughout the greater part 

 of the follicle. The outer or " parietal/' and the inner or 

 "chief," varieties of these may be recognised, but these 

 will be better seen in the osmic acid stomach. 



Bring into view the muscularis mucosa outside the closed 

 ends of the follicles. It consists of two layers of non-striped 

 muscular fibres, bundles from both of which may be traced 

 upwards between the follicles, and may in very thin sections 

 be traced nearly to the open ends of the glands, where, 

 according to Klein, they form a transverse network between 

 the glands. 



The submucous coat consists of areolar tissue \ blood- 

 vessels may be found here and there. 



The muscular coat. The fasciculi of non-striped fibres 

 can be seen cut longitudinally and transversely. 



190. Examine (H.) a V. S. mucous membrane of cat's 

 stomach hardened in osmic acid as above described. If a 

 peptic follicle can be traced throughout its entire length, it 

 will be found that near and at the orifice the epithelium is 

 columnar, with the nuclei placed near the attached ends of 

 the cells. In about the half of the follicle nearest its closed 

 extremity two sorts of cells may be seen. A large, some- 

 what spheroidal, nucleated cell here and there placed exter- 

 nally close to the basement membrane, and within this, and 



