ii8 PRACTICAL HISTOLOGY. 



b. The blood-vessels and bile ducts may be injected as 

 directed in 343, 346. 



200. Structure. Cells (H.) a. Scrape the cut sur- 

 face of the fresh liver of rabbit or cat ; diffuse the scraping 

 in a drop of salt solution ; cover and examine. The cells 

 have one rarely two nuclei ; the protoplasm is distinctly 

 granular, and there is no envelope. 



b. Prepare in the same manner the cells of the liver of 

 the ox. Particles of fat are usually to be seen in these. 



20 1. Thin section of liver of cat hardened in Miiller's 

 fluid and spirit ( 199, a). The section should be made 

 across one of the larger bile ducts. Stain with logwood, 

 by placing the section on a slide in a few drops of strong 

 logwood solution for two or three minutes. Wash in water ; 

 and mount in Farrants' solution, or in glycerine. 



(L.) The lobules. Observe the network of the gland 

 cells in each. If the section be across the long axis of a 

 lobule, branching columns of cells may be seen radiating 

 from the centre. A network of capillaries occupies the 

 spaces between the cells. The T. S. of one of the larger 

 bile ducts may perhaps be seen, with a section of the 

 hepatic artery and portal vein near it. If there be a T. S. 

 of the bile duct in the preparation, it is convenient to place 

 it exactly in the centre of the field, otherwise it will not be 

 readily found with H. 



(H.) The bile duct lined by a layer of columnar epithe- 

 lium. There is nothing remarkable about the sections of 

 the hepatic artery and portal vein, 



The capsule of Glisson. A fine fibrous tissue that sur- 

 rounds all the vessels and runs between the lobules. The 

 whole liver is enclosed in a thin fibrous capsule. The tissue 

 now seen is a prolongation of the capsule around the vessels 

 that enter at the portal fissure. The capsule also sends fine 

 trabeculae between the lobules from many points of the sur- 

 face. Look for these near the margin of the section. 

 (They are most readily seen, however, when the cells have 

 been pencilled away.) 



The columns of cells are easily seen, and in the intervals 

 between the columns, collapsed capillaries with blood cor- 

 puscles. 



