I 4 2 PRACTICAL HISTOLOGY. 



d. The lymphatics of the testis may be readily injected by the 

 method described in 344. 



250. Structure. a. T. S. testis of cat hardened in 

 osmic acid and alcohol ; mount in glycerine. 



(L.) Tunica albuginea ; mediastinum and septa passing 

 from it to the tunica. Tubuli seminiferi. 



(H.) Nucleated cells the spermatoblasts, and perhaps 

 also spermatozoids, within the tubuli seminiferi. The mem- 

 brane of the tubules is thick, and, according to Mihal- 

 kovics, consists of several layers of flattened cells, that are 

 unaffected, and may, indeed, be demonstrated by a twelve 

 hours' immersion in strong hydrochloric acid. 



b. Examine (L.) a. V. S. testis of cat, prepared as above, 

 showing tunica albuginea, tubuli seminiferi, tubuli recti, 

 rete testis, and perhaps also the vasa efferentia, passing to 

 the epididymis. 



c. Examine (H.) a preparation of spermatozoids of ox 

 or sheep, preserved by drying. 



251. Prostate. This may be hardened in a \ per cent chromic 

 acid, followed by alcohol. 



FEMALE ORGANS. 



252. Uterus. This may be readily hardened in a 

 \ per cent chromic acid followed by alcohol ( n). T. S. 

 uterus of cat, unstained ; mount in glycerine. (L.) Mucous 

 membrane and muscle. (H.) The uterine glands ; tubular, 

 and lined by columnar epithelium. 



253. Ovary. Methods. The ovary maybe exceedingly 

 well prepared by hardening in Mutter's fluid and alcohol, as 

 directed in 12 A. The sections are best 'stained with 

 logwood, and may be mounted in glycerine or in dammar. 



254. Structure, Examine a section of the ovary pre- 

 pared as directed above. 



(L.) Graafian follicle surrounded by a fibrous stroma. 



(H.) The following parts may be recognised from within 

 outwards in a mature Graafian follicle: i. The germinal 

 spot ; a nucleolus. 2. The germinal vesicle ; a nucleus. 

 3. Granular protoplasm ; the vitellus. 4. The zona pellu- 



