i8p PRACTICAL HISTOLOGY. 



shaking of the mixture, then add the remainder of solution 

 A, and also i oz. rectified spirit. The injected tissues are 

 preserved in acid glycerine (glycerine i oz., hydrochloric 

 acid two drops). 



Prussian blue injections must not come in contact with 

 alkali. The above injection masses are excellent when it 

 is of no moment to have the vessels fully distended, but 

 merely to have the indication of pigment within them, to 

 aid in their recognition amidst other tissue elements. 



334A. Asphalt and Chloroform. Ludwig has recently 

 employed a mass, consisting of asphalt dissolved in chloro- 

 form and filtered, for the injection of the bile ducts. The 

 merit of this fluid is, that chloroform being an extremely 

 mobile fluid, flows readily along the vessels, and that it 

 rapidly evaporates and leaves them filled by a solid black 

 mass. 



335. Alcannin and Turpentine. A solution of al- 

 cannin in turpentine or in chloroform is used by Ludwig 

 for injecting lymphatics. The solution is of a bright red 

 colour. Both the turpentine and the chloroform solutions 

 flow readily. When the latter is employed, the chloroform 

 evaporates and leaves the alcannin in the vessels. 



336. Solution of Silver Nitrate. The employment 

 of this has already been referred to in 3 2 9. 



2. INJECTION MASSES SOLID AT THE ORDINARY 

 TEMPERATURE. 



337. Carter's Carmine and Gelatine Mass. This 

 the best of all the carmine masses is prepared thus : 



Carmine, 60 grains. 



Strong ammonia, 120 minims. 



Glacial acetic acid, 86 minims. 



Solution of gelatine (gelatine i, water 6 parts), 2 oz. 



Water, ij oz. 



Dissolve the carmine in the ammonia and water with the 

 aid of gentle heat, and filter ; add to this i^ oz. of hot 

 gelatine solution, and mix thoroughly. Add the acid to 



