13 



The sections were designated as I, II, III and IV, beginning 

 with the outer part. The sap was collected from the different 

 spouts during the same length of time. 



The innermost, section IV, never bled sufficiently to yield a 

 sample for analysis, although the sap repeatedly stood in the 

 end of the tube. This may have been due to the size of the 

 hole but a one-fourth-inch hole will bleed freely as shown in 

 Bulletin 24. 



The relative variations in sap-flow from I, II and III were 

 much alike until April 12, when the outer sections stopped 

 bleeding and the flow from III reached the maximum. 



In this respect number 10 yielded results very similar to 

 number 12. In composition, however, it did not agree with the 

 other two trees, but after the first two days, the inner portion 

 yielded the richest sap. 



The buds burst and the leaves began to expand before the 

 sap ceased to flow from section III. 



