NON-SPORING BACILLI 245 



and at times is intracellular. It is extremely small and 

 ovoid, so that it is readily mistaken for a micrococcus. 

 It is slightly larger than the bacillus of influenza, and 

 more ovoid. It is Gram-negative, stains with Loeffler's 

 methylene-blue, dilute carbol-fuchsin, or aqueous fuchsin. 

 Toluidin blue in a special medium is advised. (Toluidin 

 blue 5 grm., alcohol ioo c.c, water 500 c.c. ; dissolve ; 

 add of 5 per cent aqueous carbolic acid solution, 50 c.c. ; 

 stand two days ; filter.) 



Bacillus of Ducrev Is a small bacillus (1 to 2 micra 

 X 0-5 micron), regularly found in soft chancre or chancroid. 

 It is non-motile, non-flagellar, non-sporing, Gram-negative, 

 and is found at times inside the leucocytes. 



Culture. It only grows on whole blood agar, and dies 

 off at room temperatures. Colonies show in about 48 

 hours. Inoculation of pure cultures on the skin produces 

 typical chancres in 4 to 6 days. 



Zur Nedden's Bacillus. A small slightly curved 

 bacillus (1 micron long) which has been isolated in ulcera- 

 tive conditions of the cornea. It grows well on the 

 ordinary media. It is non-motile, Gram-negative, and 

 does not liquefy gelatin. It clots milk, forms acid with 

 glucose, but no indol in peptone water. 



Culture. It grows well on potato. 



MORAX-AXENFELD DIPLO-BACILLUS. 



This is a short, thick bacillus with rounded ends, found 

 in a chronic eye condition called " angular conjunctivitis," 

 in which there is slight redness of the edges of the lids, 

 especially at the angles. The ocular conjunctiva is seldom 

 affected. It mainly affects adults, and chiefly women. 

 There is rarely any corneal trouble. It was described by 

 Morax in 1896, and by Axenfeld a year later. The diag- 

 nosis is easily made, by taking a smear of the small bead 

 of pus which gathers through the night at the angles, and 

 staining with the usual dyes. The bacillus is easily stained, 

 and is Gram-negative. In stained films, short and long 

 chains of polar stained bacilli are seen. 



Culture. It grows best on Loeffler's blood serum at 

 37 C, causing small pits of liquefaction in 48 hours. 



