284 PUBLIC HEALTH BACTERIOLOGY 



or inoculate guinea-pig. In urine : sediment or centri- 

 fuge, make films, and examine. To avoid smegma bacilli, 

 take specimen after cleansing meatus, or by catheter, 

 and in staining decolorize with absolute alcohol after 

 the acid (see pp. 163 and 285. In pus, faeces, and tissues : 

 dissolve in antiformin as above, and examine deposit. In 

 milk : centrifuge, take sediment and fat, and mix, make 

 films, fix, remove fat with ether (not absolutely necessary), 

 stain, and examine. Also inject o-i c.c, 1 c.c. and 3 c.c. of 

 mixed sediment and fat into three guinea-pigs respectively ; 

 kill in three weeks and examine peritoneum, post-sternal 

 glands, pancreas, spleen, and liver. 



2. Inoculation. Select a guinea-pig and inject, sub- 

 cut aneously or intraperitoneally, the fluid to be tested, or 

 the washed sediment from an antiformin solution of tissues, 

 faeces, or sputum. The animal usually dies in six weeks, 

 if tubercle bacilli are in the injected substance, with local 

 and glandular changes, the spleen showing numerous 

 tuberculous nodules and being swollen as a whole. 



3. Cultivation Is made from the inoculated animal, or 

 from antiformin solution sediments, or from sputum 

 treated with 2 per cent ericolin. 



4. Tuberculin Reactions. 



OTHER ACID-FAST BACILLI. 



Besides the bacilli of human, bovine, avian, and fish 

 tuberculosis, there are other bacteria which are acid- 

 fast, as already enumerated on page 163. Such are : 

 Moeller's Timothy - grass bacillus I (from infusions of 

 Timothy grass) , Moeller's Timothy-grass bacillus II (from 

 the dust of a hay-loft), butter bacilli (isolated from butter 

 by Petri, Rabinowitch, Korn, Tobler, Coggi, and others), 

 mistbacillus (from dung by Moeller) . All these are mor- 

 phologically very similar to the tubercle bacilli, are ex- 

 tremely acid-fast, and produce lesions in guinea-pigs on 

 injection, which closely resemble tubercles. They are, 

 however, easily distinguished by their rapid growth on 

 ordinary media, colonies being visible in twenty-four hours 

 at 37 C. (in the case of the tubercle bacillus, the earliest 

 is eight days), and by their growth in most instances at 



