YEASTS AND MOULDS 339 



administered. When iodide was given, cure was often 

 effected, and so the diagnosis was apparently confirmed. 

 The ulcers have similarly been treated as syphilitic, as 

 lupus, and as simple pyogenic ulcers. In many cases 

 there is a history of a minor injury, with a spread from 

 this up the line of the lymphatics, with tumour formation 

 and breaking down at various points en route. The 

 lymphatic glands are not usually enlarged, the fungus 

 probably not reaching them at an early stage. The 

 affection is usually not a serious one, but there is little 

 tendency towards cure if left untreated. Stimulating 

 local treatment, together with the administration of large 

 doses of potassium iodide (60 to 80 grains, or 4 to 5 grm. 

 daily) is quickly followed by cure. The diagnosis is based 

 on the direct examinations of scrapings, which are usually 

 negative except for spores, oval and 3 to 5 micra long ; 

 and by cultivation. Pus from an unbroken abscess (if 

 possible) is inoculated freely, since the parasitic elements 

 are scarce, on broth, glycerin agar, potato, carrot, etc., 

 on all of which it grows well. Growth becomes visible 

 in some days, and gradually increases. On agar, the 

 colonies are first white and cream, and later a dirty grey. 

 On carrot, the colour is first yellow, then grey, and finally 

 quite black. On potato, small, white, woolly spots 

 appear, increase in size, and change to a brownish colour. 

 Further growth results in heaped-up masses likened to 

 cerebral convolutions. " In gelatin stab, an inverted 

 fir-tree growth is got, but no liquefaction. It forms acid 

 specially with inulin in peptone solution, and also with 

 glucose, maltose, galactose, raffinose, saccharose, and 

 mannite ; but with lactose, dulcite, inosite, adonite, 

 sorbite, and salicin, the medium remains alkaline. In 

 no case was there any gas formation. No indol formation 

 was observed. The organism was definitely aerobic " 

 (Ritchie, loc. cit.). This observer has also studied the 

 organism in hanging-drop agar cultures, and found that 

 the mycelium formation is readily noted at 22 C, but at 

 37 C. few filaments are formed ; instead, large spores 

 (5 micra) from which short stalks sprouted, each bearing a 

 spore, which thus formed a circle round the central body. 

 The latter soon degenerated. This suggests the reason 



