Introductory. 



for working purposes only. For the chief tests 

 employed they are as follows: — 



^-*«-- 5 I Agar at 37 deg. 0. ( "^^^l,, ,.„. 



R --ij (less than 



^■"^^^ U,000 per C.C. 



*B enteriditis sporogenes test andw . 



2?di. CO ^^^^"'^^'^"'^ ^"""^ **3 with 0-1 C.C. 



Indol test (five days at 37 deg. C.)" 

 Neutral-red broth test (two days at 



37 deg. C.) Negative result 



Bile- salt broth test (two days at f with 



37 deg. C.) 0-001 c.c. 



Litmus milk (modified) test (two days 



at 37 deg. C.) 



" These are primary standards ; my secondary 

 standards are arrived at by rendering the primary 



* " It will be seen from the resulta detailed in Appendix VH. that, in 

 connection with the routine use of the B. enteriditis sporogenes test, 

 the habitual inoculation of rodents is neither necessary nor wholly 

 justifiable." 



standards ten times more lenient." (Part III., 

 pages 12 and 13.) 



Dr. Houston, in the introduction to Part III. 

 (Bacteriological Report), draws special attention to 

 the necessity for distinguishing between efiBuents 

 that are to be discharged into drinking-water and 

 into non-drinking-water streams. In this con- 

 nection the protection of shell-fish and watercress 

 must also be borne in mind. It may be of interest 

 to state here that diiring convalescence typhoid 

 bacilli pass out of the body by way of the kidney 

 and urine in enormous numbers — up to 6,000 mil- 

 lion per cubic centimetre. (Dr. Horton Smith, 

 Gulstonian lectures before the Royal College of 

 Physicians, 1900; quoted by Prof. Klein on 

 page 10; Experiments and observations on the 

 vitality of the bacillus of typhoid fever and of 

 sewage microbes in oysters and other shell-fish — 

 investigations on behalf of the Worshipful Com- 

 pany of Fishmongers — May, 1905.) 



