PLATE. 



XI. 



THE HISTOLOGICAL TECHNIQUE 



HARDENING FLUID ; TIME OF HARDENING. 

 Berkley (used) 2 hrs., osmic-bichromate 50 hrs. 



II 



XII. 



XIII. 



XV. & XVI. 



XVII. & XVIII. 



Osmic-bichromate + 2\% of formalin (unnecessary) 18 hrs. 



Osmic-bichromate (used) 4 da. 15 hrs. 



Lithium bichromate 2\<J 3 da. 3 hrs. 



Potassium bichromate 5% '+ 5% of formalin, 22 hrs. 



XX., XXII. to ) 



> Osmic-bichromate 6-J- da. 

 XXXII., inc. \ 



XXI. 



XXXIII., XLII. to ) 



Osmic-bichromate 2 da. 



Osmic-bichromate 5 hrs., Berkley's solution 6 da. 

 XLIV., inc. > 



XXXV. & XXXVI. Osmic-bichromate 5 hrs., Berkley's solution 6 da. 



XXXVIII. 



Osmic-bichromate 2 hrs., Berkley's solution 2\ da. 



XXXIX., XLV. to) 



\ Osmic-bichromate 7 da. 20 hrs. 

 XLVIII.,inc., L. \ 



XLI. 



LI. 



Osmic-bichromate 2| da. 

 Osmic-bichromate 6 da. 4 hrs. 



,. , 



i vol. ) 



SILVER SOLUTION. 

 (Silver nitrate 2% i vol. 

 ( Sodium sulphate 2 % 



Silver nitrate i <f c 



Silver nitrate \% 



Silver nitrate i % 



Silver nitrate i % 



Silver nitrate | % 

 Silver nitrate |% 



Silver nitrate f % 



(Silver nitrate 2% i vol. ) 200 cc. -f- 2 drops 

 ( Zinc sulphate 2 % i vol. ) formic acid. 



Silver nitrate f % 



Silver nitrate f % 

 { Silver nitrate 4% i vol. 

 ( Zinc sulphate 10% i vol. 1 



( Silver nitrate 2 % i vol. ) 200 cc. + 3 drops 



x > 



( Zinc sulphate 2% i vol. ^ formic acid. 



The Golgi method, like all special methods, has its peculiar defects, which are liable to 

 lead to misinterpretation. If, however, familiarity be acquired with the various appearances 

 it gives, the careful investigator will not be misled by irregular precipitations, etc., any more 

 than he is in other directions when using other special methods. Its great value in the 

 investigation of nervous histology cannot but be admitted by all. 



