PLATE 36. 



Bacillus anthracis. F. Cohn and R. Koch. 

 (Splenic Fever.) 



I. Smear preparation from the blood of the spleen of a 

 mouse. X 1000. 



II. Contact preparation of an agar plate culture, one day 

 at 22. X 1000. 



III. Unstained preparation in hanging drop from a 'bouil- 

 lon culture, thirty-six hours at 37. X 1000. Spores begin 

 already to escape. 



IV. Anthrax threads from agar, thirty-six hours at 37. 

 X 1000. Stained with ZiehTs solution; spores red, bacilli 

 blue. 



V. Involution forms. Five-weeks'-old agar stab cul- 

 ture, stained with fuchsin. X 1000. 



VI. Unstained preparation in hanging drop from a bouil- 

 lon culture, eight hours at 37. X 1000. Beginning of 

 spore-formation. 



