SECTION CUTTING 13 



the oil of cloves. The melted paraffin should then be washed 

 off by turpentine, when the sections will remain fixed to the 

 slide by the collodion, and may be mounted in balsam in the 

 usual manner. 



Instead of the mixture of collodion and oil of cloves, a solution 

 of shellac in absolute alcohol may be used : this should be 

 spread over the slide in a thin layer by means of a glass rod, 

 and allowed to dry. Immediately before being used the slide 

 should be brushed over with oil of cloves. 



If it is desired to stain the sections on the slide, the thinnest 

 possible layer of glycerine and albumen (pure glycerine and 

 fresh white of egg in equal parts, filtered) should be smeared 

 over the slide, and the sections laid on it in order. The 

 paraffin, when melted, should be washed off with turpentine or 

 benzole, and the sections dehydrated with absolute alcohol ; tho 

 desired stain can then be used in the usual manner. Dehydrate 

 with absolute alcohol, followed by oil of cloves, before mounting 

 in Canada balsam. 



