34 CONDITIONS OF BACTERIAL LIFE. 



Water, 1000. Magnesium sulphate, 0.2 to 0.4. 



Glycerin, 30 to 40. Di-potassium phosphate, 3 to 2.5. 



Sodium chlorid, 5 to 7. Ammonium lactate, 6 to 7. 



Calcium chlorid, 0.1. Sodium asparaginate, 3 to 4. 



Instead of this complicated solution, one may employ 

 many simpler ones; for example, such as is recommended 

 by Voges and C. Frankel (Hyg. Rundschau, 1894, No. 

 17, 769), which is as follows: 



Water 1 liter. 



Sodium chlorid 5 gm. 



Neutral commercial sodium phosphate ... 2 gm. 



Ammonium lactate 6 gm. 



Asparagin 4 gm. 



Upon this (although there is no sulphur in the nutrient 

 medium) the following grow: 



VERY WELL: FEEBLY: 



Bac. subtilis and mycoides, Mic. pyogenes a aureus, 



Bact. syncyaneum, pyocyaneum, Streptococcus pyogenes, 

 coli, acidi lactici, pneumonias, Bact. typhi, 

 mallei, vulgare, Bac. anthracis. 



All vibriones. 



NOT AT ALL: 

 Bac. tetani, 

 Bact. murisepticum, 

 Bact. erysipelatos suum, 

 Bact. cuniculicida. 



Even with the addition of those substances recommended 

 by Uschinsky, other varieties, as diphtheria l and tetanus, 

 did not grow luxuriantly, but, by adding 3% to 4% of gly- 

 cerin, the medium can be used for cultivating many vari- 

 eties, even the tubercle bacillus. 



While cultures upon the simple nutrient media just de- 

 scribed possess a great theoretical interest, yet they have 

 been but little employed for diagnostic purposes. 



Very much more use is found for meat-infusion, peptone- 

 gelatin, agar, and bouillon (each with or without the addition 

 of grape- or milk-sugar}, also glycerin-agar, milk, and slices 

 of potato. (For preparation see Technical Appendix.) 



We must always keep these on hand, since without them 



1 Recently Uschinsky has apparently obtained upon his non-albu- 

 minous nutrient medium a good growth with the production of toxin 

 in the case of a certain culture of diphtheria. 



