SABCINA LUTE A. 157 



Sarcina lutea. 1 (Flugge, emend. Lehmann and 

 Stubenrath). 



Microscopic Appearance. Upon nutrient media typi- 

 cal bales of packets. 



Gelatin Plate. (a) Natural size. Roundish, puncti- 

 form colonies, sulphur-yellow; after ten to twenty days, 

 sinking in (3, v). 



(b) Magnified fifty times. Superficial: Roundish, even- 

 bordered or almost smooth-edged colonies; pale yellow 

 with at first a finely granular and later (eight to ten days) 

 a more coarsely granular structure. After a very long 

 time the peripheral parts separate somewhat and, with 

 higher magnification, individual tetrads are seen (3, vi). 

 Deep lying : Roundish, dark yellow, even- bordered, finely 

 granular. 



Gelatin Stab. Stab : Thread-like, with relatively few 

 coarse granules. Surface growth : Irregularly circular, with 

 a moist luster, somewhat elevated, sulphur, lemon-, or 

 even deep yellow. After ten to twelve days the superficial 

 growth sinks down. Liquefaction at first extends in a 

 funnel form and later as a cylinder; however, we have 

 cultivated almost non-liquefying forms (3, i). 



Agar Plates. (a) Natural size. Superficial: Round 

 or roundish, even-bordered, somewhat elevated; sulphur- 

 yellow, with a moist luster. Deep : Roundish to whet- 

 stone-shaped (3, vii ). 



(6) Magnified fifty times. Superficial: Roundish almost 

 even-bordered colonies; periphery delicately punctate; 

 peripheral zone transparent, pale yellowish, becoming 

 darker toward the center; finely to coarsely granular 

 (3, vm). Deep: Like those upon gelatin with coarser 

 granulation. 



Agar Stab. Stab : Thread-like, finely to coarsely gran- 

 ular, at times after a long while ray-like outgrowths ; yel- 

 low. Surface growth : Roundish, wavy, even border, some- 



1 Plate 6, Figs. I to v, illustrating the Micr. luteus Cohn, serve ex- 

 actly as well for the Sarc. lutea, except figure in, where the bales of 

 packets are absent. Also Plate 3 would pass for the gelatin plate cul- 

 tures, except for the finely granular structure (3, vin) ; a somewhat 

 lighter form (5, iv). 



