308 IMPORTANT VARIETIES OF FISSION-FUNGI. 



Motility. Always is absent. To this no exception is 

 known. 



Staining Properties. Stains with all anilin dyes and 

 by Gram's method. 



Relation to Oxygen. Grows best when oxygen is 

 admitted. When oxygen is excluded, it grows poorly 

 and without liquefaction. There is no growth in CO 2 . 



Intensity of Growth. Grows rapidly, especially at 

 37. Lower limit of growth at 14 (Kitasato). 



Gelatin Plate. (a) Natural size. Superficial colony : 

 Whitish, round; after three or four days, deeply sunken. 

 Also, upon longer standing the liquefaction only extends 

 slowly. In the middle of the abrupt crater there lies a 

 white, crumbly, poorly defined mass, the remainder of the 

 contents of the liquefied area being rather clear, but the 

 outermost peripheral zone is somewhat turbid again (35, v). 



(6) Magnified seventy times: The colonies when three 

 days old appear distinctly darker than on agar. Near the 

 center grayish-yellow, toward the edge more distinctly 

 transparent. At the periphery the formation of locks 

 is clearly seen, but toward the center they become very 

 dense and indistinct (36, vi). The liquefaction is recog- 

 nized as a grayish reflex. Later an irregularly outlined 

 ball, devoid of distinct locks, floats in the liquefied 

 medium. 



Gelatin Stab. Along the stab there forms a thick 

 white thread, from which, as a rule, only in the upper part 

 (34, n), more rarely throughout the entire length, long 

 (34, i) or short (34, in), bristly, distinct outgrowths 

 extend outward. Sometimes the growth of hairs fails 

 entirely (34, iv). Also the direction of the lateral out- 

 growths varies; many times they are tangled together 

 (34, v). After twelve to twenty hours there begins a 

 slowly progressing liquefaction, with limited depression of 

 the surface of the gelatin. The liquefaction at first is 

 cup-shaped, later cylindric. The content of the funnel 

 is sometimes diffusely cloudy with white crumby flocculi; 

 at other times the flocculi settle down, leaving a clear 

 liquid gelatin above. No pellicle is ever formed. 



Agar Plate. (a) Natural size. Superficial colonies: 

 Small, white, with a play of yellow, moistly shining, a 



