334 IMPORTANT VARIETIES OF FISSION-FUNGI. 



Gelatin Stab. Deep in the gelatin along the stab 

 there occurs first a cloudy growth, then cyst- or tube- 

 shaped excavations, which are filled with cloudy, granular 

 liquid (44, n). 



Agar Plate. (a) Natural size : Colonies whitish, 

 roundish to ragged, usually surrounded by an exceedingly 

 delicate veil (44, v). 



(b) Magnified sixty times: The original colony appears 

 grayish-yellow, roundish, opaque, surrounded by a broad 

 zone made up of the finest interlacing hairs. Toward the 

 periphery it becomes transparent; toward the center, 

 grayish-yellow and opaque (44, vi). 



Agar Stab. In the stab prepared by a simple thrust 

 of the platinum loop there develops, deep in the agar, a 

 scaly, ribbon-shaped growth (compare 45, n). If one 

 rotates the loop in the agar, then the growth extends in a 

 wider zone and consists of a cone of cloudy layers (44, i), 

 the surface of which, after a very long time, becomes 

 covered with points and fine serrations (45, in). 



Agar Streak. No confluent growth, but only single 

 discrete colonies (Votteler). 



Blood-serum. Sometimes liquefied, sometimes not. 



Bouillon (anaerobic) moderately clouded. 



Milk. No (according to v. Hibler, very slow) coagu- 

 lation; reaction amphoteric. 



Non-albuminous Nutrient Media. Upon Uschin- 

 sky's solution no distinct growth. 



Resistant properties of the bacillus are without 

 practical interest, as it sporulates very readily. 



Resistance of the Spores. See page 331, andTizzoni 

 and Cattani (C. B. ix, 487). 



Chemical Activities. See page 331. The forms 

 studied by us form gas from sugar actively; a production 

 of acid could not be demonstrated (on account of simul- 

 taneous abundant formation of alkali). Brain nutrient 

 medium was darkened (v. Hibler). Extremely vigorous 

 production of H 2 S, little indol. Attenuated (slightly 

 virulent) forms, according to Tizzoni and Cattani, often 

 form more acid and grow more luxuriantly (C. B. xi, 150); 

 in general, the virulence is well preserved. Upon nutrient 

 media without sugar, we saw no gas production. The" 



