32 Experiment Station Bulletin 363 



were cured. The cases of streptococcal or staphylococcal mastitis not 

 cured by the first administration of penicillin were those given one or 

 two injections of 100,000 units of penicillin per 100 ?nl. dose. No mas- 

 titis streptococci or staphylococci could be detected in any of the cows 

 considered cured when quarter samples were tested at weekly intervals 

 over a period of four to five weeks after treatment. It was found that 

 the organisms were eliminated from the udder when tested three to seven 

 days after treatment, and that the leucocytes also disappeared even in 

 cows showing leucocyte counts of 20,000,000 or more per ml. before 

 treatment. 



These results indicate that penicillin is highly effective for the treat- 

 ment of bovine mastitis. Streptococcal mastitis may be cured by one in- 

 jection of 100,000 Oxford units of penicillin administered in 100 ml. of 

 sterile saline or distilled water through the teat canal. In more acute 

 cases of long-standing, chronic infection, one or more injections contain- 

 ing 200,000 units may be necessary. Straphylococcal mastitis is more 

 difficult to cure and may require one or more treatments with penicillin 

 containing 200,000 Oxford units per 100 7;//. dose. 



These studies are being continued to determine the maximum dose 

 of penicillin necessary for curing streptococcal and staphvlococcal mas- 

 titis, the best method of administration and the value of penicillin for 

 the treatment of acute cases. 



In collaboration with Cerbiniol Laboratories of New York, the chem- 

 otherapeutic value of cerbiniol for mastitis was also studied. Approxi- 

 mately 50 cows with streptococcal mastitis and 1 1 cows with straphylo- 

 coccal mastitis were injected intravenously with this drug. The results 

 to date indicate that cerbiniol is not effective for the treatment of masti- 

 tis when injected in this manner. 



L. W. Slanetz, F. E. Allen, T. Sicilian 



FORESTRY 



Study of Sugar Maples 



Two small lots of seed, one from Sweet Sue (maple on the campus 

 of the University of New Hampshire) and the other from a known sweet 

 tree in Dublin were sowed in a seed-bed in the nursery on October 26, 

 in order to test fall planting as a method of storing sugar maple seed. The 

 seed germinated early and well, and the seedlings are now growing 

 vigorously. 



Nineteen grafts were made on small maples, 1 1 with cuttings from 

 Sweet Sue, and eight from other maples. On iVIay 29, only one graft 

 looked as if it might succeed. The large pith present in the young 

 twigs apparently was a complicating factor in making successful grafts. 



Experimentation with indoor and outdoor beds for rooting cuttings 

 \^'as continued and some new phenoxy growth compounds were tested. 

 The results are still unsatisfactory, as the percentage of cuttings which 

 develop roots remains low. About 50 of the rooted cuttings were set in 

 pots, placed in a coldframe, and mulched with straw over winter. They 

 survived the winter, and 36 were set out on April 20, some in a maple 



