28 Experiment Station Bulletin 367 



and energy balances were run on each group after definite deficiency 

 symptoms were observed in the animals not being fed supplemental Vita- 

 min D. 



A significant difference in the utilization of protein or energy was 

 not detected by means of "balance" studies. However, some of the defi- 

 cient animals were so rachitic that they would not consume a normal 

 amount of feed while "balance" studies were in progress. This may have 

 masked any differences which otherwise would have been observed had 

 they been eating normally. Although the balances showed negligible 

 differences, growth studies showed a greater and more efficient gain by 

 both animals which received supplemental Vitamin D as compared to the 

 deficient animals. 



N. F. Colovos, H. A. Keener, E. G. Ritzman 



Nutrition Studies with Lactating Cows 



In this study it was planned to include the determination of digesti- 

 bility and utilization of the protein and energy of Ladino clover pasture 

 by milking cows. 



Various stages of growth were to be used in different experiments 

 as plant growth permitted. Because of adverse weather conditions, how- 

 ever, the Ladino clover was killed out to such an extent during the spring 

 months that such experiments were not possible during the early part of 

 1946. 



N. F. Colovos, H. A. Keener 



Determination of the Ratio of Solids-not-Fat 



In Milk Produced by Cows in Different Family Lines 



Solids determinations were completed on milk from those cows in 

 the University herd that did not have two previous lactation records. 



Of the 22 sires on which records are now available, six lowered milk 

 production, increased fat percentage and solids-not-fat percentage; five 

 increased milk production and fat percentage but lowered solids-not-fat 

 percentage; three increased milk production, fat percentage, and solids- 

 not-fat percentage; three increased milk production and lowered both 

 fat and solids-not-fat percentage; two increased milk production and 

 solids-not-fat percentage but lowered fat percentage; two lowered milk 

 production and solids-not-fat percentage but increased fat percentage; 

 and one lowered milk production slightly and lowered the fat percentage 

 but increased the solids-not-fat percentage. 



H. C. Moore, H. A. Keener 



Relationship of the Composition of Milk 



To Methods Used for Determning Adulteration 



Progress on this project was seriously retarded due to temporary 

 leave of absence of one of the personnel. Some work was done on stand- 

 ardizing methods, although no actual experimental results were obtained 

 with individual cow samples or field samples. Solids analyses were con- 

 tinued on cows in the University herd on which records for two lacta- 



