52 



51. Bacillus esterigenes; 53. Bacillus esterigenes A; 54. Bacil- 

 lus esterigenes D; 68. Bacillus fluorescens tenuis; 70. Bacillus 

 fuchsinus; 71. Sarcina gasformans; 72. Bacillus graviolens; 

 80. Bacillus hyponitrous; 87. Bacillus juglandis; 88. Bacillus 

 kiliensis; 92. Bacillus levans; 96. Sarcina liquefaciens; 97. 

 Bacillus liquefaciens; 102. Micrococcus luteus; 103. Sarcina 

 lutea; 104. Streptococcus luteus liquefaciens; 105. Bacillus 

 maidis; 111. Bacillus megatherium; 113. Bacillus proteus 

 mirabilis; 116. Bacterium muris; 119. Bacillus ochraceus; 

 122. Oidium lactis; 124. Cladothrix odorifera; 125. Cladothrix 

 dichotoma; 130. Bacillus Petasites; 135. Bacterium phyto- 

 phtorum; 137. Bacillus pumilis; 139. Bacillus fluorescens 

 putidus; 142. Bacterium radiatum; 144. Bacillus ramosus 

 non liquefaciens; 145. Bacillus rosaceus; 155. Bacillus rubidus; 

 156. Spirillum rubrum; 159. Spirillum Rugula; 160. Bac- 

 terium rubilum; 161. Bacillus ruminatus; 164. Spirillum 

 serpens; 169. Bacterium luteus sporogenes; 172. Bacillus 

 sphaericus; 174. Staphlococcus pyogenes citreus; 178. Bac- 

 terium subflavum; 179. Micrococcus sulfur; 184. Bacillus 

 tumefaciens; 185. Bacillus tumescens; 194. Bacillus vivax; 

 200. Bacillus Zopfii. 



Of the 201 organisms under consideration 139, or 69.1%, 

 reduced nitrate to nitrite, and 62, or 30.9%, did not effect 

 this reduction. Those organisms which produce green pig- 

 ment almost invariably reduce nitrate to free nitrogen. This 

 reduction takes place very rapidly, after forty-eight hours 

 no nitrite remains in solutions of small concentration. It is 

 impossible to declare from the vigor of the growth of the 

 organism respecting its ability to effect the reduction of nitrate 

 to nitrite. However, those bacteria which failed to perform 

 such reduction were commonly found among those whose 

 growth was slow and at best feeble. 



Many organisms were inoculated into Giltay's medium in 

 the hope that it would prove available for reduction experi- 

 ments. This proved to be the case with soil inoculation, but 

 in pure culture the slow growth of all and the refusal of many 

 bacteria to develop in this synthetic medium, did not prove 

 encouraging. Calcium glycerophosphate and calcium lac- 

 tophosphate were also tried in this connection. These com- 

 pounds proved to be unstable in solution and were difficult 



