420 APPENDIX. 



but to little other disturbance. Like the Staphylococcus pyogenes albus 

 appears to be associated with suppurative processes and is found under 

 similar conditions. 



(2) Staphylococcits pyogenes citreus. Found in the pus of acute abscesses ; 

 differs from No. I only in the fact that instead of being dark orange yellow 

 it remains bright citron yellow in colour. 



(3) Diplococcus subflavus. Grows rapidly on nutrient jelly and blood 

 serum, first as whitish points which gradually become yellowish and then 

 deep yellow ; in large quantities produces abscesses ; occurs in several 

 secretions as a diplococcus from 0.5 to 1.5/1 in diameter ; it is made up of 

 two hemispheres with a central division and resembles the gonococcus 

 somewhat in appearance, but retains the aniline dyes much more tenaciously 

 than that organism. 



(4) Streptococcus coli gracilis, Occurs in the intestinal canal and faeces 

 of the carnivora ; on plates it forms small sharply outlined dark colonies 

 in the centre of an area of clear liquefied gelatine ; these, later, become 

 somewhat crenated at the margins ; in a gelatine tube the medium is 

 liquefied rapidly along the track of the puncture and after six or eight days 

 there is precipitated a white finely granular mass ; on agar-agar, potatoes 

 and blood serum there is very little superficial growth even at the body 

 temperature, which is most favourable to its growth ; it is a coccus from 

 .2 to .4/1 in diameter; in fresh gelatine cultures it forms curved chains 

 consisting of from six to twenty cocci. 



/3 The colonies are found occupying both the centre and periphery of 

 the liquefying area. 



(1) Micrococctis coronatus. Appears on the second day in plate cultivations 

 as whitish yellow points ; deep colonies, under the microscope appear as 

 opaque sharply-defined plates; superficial growths project slightly, this is made 

 more marked by a slight zone of depression surrounding the gelatine ; at 

 intervals tooth-shaped processes advance beyond the general circular peri- 

 phery ; the older growths are dark in colour, newer growths are yellow or 

 yellowish brown ; liquefaction takes place around the growth in presence 

 of air ; the coccus, i.i to i.2ju in diameter, occurs singly in short chains or 

 in irregular masses. 



(2) Micrococcusradiatus. Growths visible in twenty-four hours ; I mm. in 

 diameter in two days ; white or yellowish green, sharply defined, granular, 

 or with outgrowths like the rays of a star fish ; colonies sink as gelatine be- 

 comes liquid, and a series of circles of rays formed of delicate threads project 

 radially, this zone increasing in breadth towards the periphery ; one, two or 

 three of these circles are seen according to the age of the growth, the 

 rays of the outer circles always being shorter than those of the inner ones, 

 each circle forms in about two days ; in a puncture cultivation isolated 

 points form along the track of the needle ; from these, lateral branches 

 project ; a funnel-shaped area of liquefaction is formed very slowly, it 

 extends for a short distance only into the gelatine ; micrococci .8 to .9/1 in 

 diameter ; usually grouped in small masses but sometimes in short chains. 



(3) Micrococcus flavus liquefaciens. Occurs on gelatine plates as small 

 yellow circular, oval, or irregular finely-toothed colonies ; superficial colonies 

 distinctly yellow, cause liquefaction. Smaller colonies are found at the 

 border of the liquefying area which has a very sharp outline ; lines of cocci 

 radially disposed run from the centre to the periphery in the clear liquefying 

 area, giving an appearance that is said to resemble the wheel of a waggon ; 



