334 APPENDIX. 



mould colony and examine its structure with different powers 

 of the microscope. Make a set of drawings showing the 

 details of structure and the mode of reproduction in the 

 specimen that you have. 



EXPERIMENT 5 : (a) Determine the motility of the various 

 growths of bacteria on potato in the Petri dishes. 



(6) Make four permanent slides from different appearing 

 growths, stain with methylene-blue and examine microscopi- 

 cally. Make a drawing of each, showing their structure. 



EXPERIMENT 6 : Staining of Spores: Select a culture of 

 bacteria in which spore-formation can be seen, and double 

 stain the spores and bacteria (carbol-fuchsin and methyl-blue). 



EXPERIMENT 7 : Colony Culture: (a) Inoculate a tube of 

 beef-tea with a portion of each of the different growths on 

 potato, (b) After forty-eight hours make a plate culture on 

 agar and an agar roll culture, inoculating each of these from 

 the beef-tea culture previously made. 



EXPERIMENT 8 : Tube Culture : Make stab and streak 

 cultures on gelatin, agar, potato, and bouillon of various differ- 

 ent appearing colonies in the plate and roll cultures. Label 

 each. 



EXPERIMENT 9 : (a) Make slides of each bacterium and 

 compare them with the slides previously made from the 

 original growths on potato. (6) Make drawings and describe 

 fully the appearance and growths of each bacterium on the 

 various media and in the colony. 



EXPERIMENT 10 : Inoculate an agar tube with a drop of 

 tap-water ; make plate culture. Cultivate each bacterium 

 present, make slides, drawings, and describe fully as in pre- 

 vious experiments. 



EXPERIMENT 11 : Study of most common non-pathogenic 

 bacteria, following out the directions laid down in experi- 

 ments as above. 



