DISTRIBUTION OF NITROGEN 109 



ogen, lactalbumin and lactoglobulin in these secretions were identical. 

 They found that colostrum in the earliest stages of secretion was very 

 rich in protein (18-20 per cent, of the total secretion), that it con- 

 tained 4-5 per cent, of caseinogen, 07-1*5 per cent, of lactalbumin, 

 and 6-12 per cent, of lactoglobulin. They separated the globulin into 

 pseudo- and eu-globulin and found these to be identical. They con- 

 sidered that euglobulin was a protein-lipoid complex as suggested by 

 Chick (see above). Lactoglobulin gave analytical data so close to those 

 given by serum-globulin [Hartley, 1914] that they were regarded as 

 identical proteins. Lactalbumin differed greatly from serum albumin. 

 The difference in chemical composition of the three proteins leaves no 

 doubt that milk contains three distinct proteins. Their figures for 

 caseinogen are very close to the figures determined by Van Slyke, only 

 that for arginine being higher. The analysis of lactalbumin is similar 

 to that by Osborne, Van Slyke, Leavenworth and Vinograd [1915]. 

 The high lysine content of lactalbumin is the most noticeable differ- 

 ence in the three proteins ; caseinogen has the least amount of mono- 

 amino nitrogen and the greatest amount of non-amino nitrogen. 



Drummond [1916] analysed a large number of normal human and 

 chicken tissues for comparison with pathological human tissues with 

 special reference to cancer. In general, the pathological tissues showed 

 a higher content of the di-amino acids and they are attributed to the 

 fact that these tissues all contain more nuclear material rather than to 

 a specific difference of cancer tissue as believed by Kocher [1915]. 

 The hexone base content of the various organs of man and other 

 animals in health and disease has been determined by Wakeman 

 [1905, I, 2; 1908] by the Kossel-Patten method. No very marked 

 differences were noticeable, but there was a slight lowering of the bases 

 in the pathological conditions. In the human liver, the amount of 

 arginine varied from 6*9 in the normal to 5*1 in the pathological, that 

 of lysine from 6*6 to 4-9, and that of histidine from 2-0 to 1-7 ; these 

 figures are in terms of the total nitrogen of the dry substance, which 

 was 1 1 '9 percent, in the normal and varied from 10*8 to 12*9 in 

 the diseased organ. For purposes of comparison of proteins this 

 method of nitrogen distribution is thus of extreme value. 



Analyses of various cattle food-stuffs have been made by Grindley, 

 Joseph and Slater [1915], Grindley and Slater [1915], and by Nollau 

 [1915]. The figures given by these workers are not always in agree- 

 ment, but are accounted for by a slight difference in manipulation. 

 Grindley and his co-workers hydrolysed the feeding-stuff and carried 



