GPM Fro’ * 
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CONTENTS. 
OUTLINE OF METHODS OF WORK. 
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aTeK ab, PR CRIAPES SS ocles 7 taies.o. saison yaeoae se ct 3 
AGES PISCASE Sos. c ocloaa ea sieicecas oseeieeiidslebetneee 4 
IPRCVsOtS LAteTaRUT Es eds occ acs e oes teoessless 6 
Geographical Distribution.................... 7 
Site OF PNRCAL OT osc soeave ered e nics aeunaase 7 
Pathological: Histology. oo... 6 cs0ces nce ees 8 
Direct-infection Experiments................. 9 
SEO OULIPRIRISUIN 5 oly. a/ 0 58-209 eve, cic a ase toeitvs-aieiclaie'pib aye 9 
PAI MONONEG 03.269, 3551 559 cD aids wan aradys eaaehee ce 9 
PEPE: OR. PEOOR stasice cies Selects cre ser smiet 9 
WOR MONOEY 9055.0 2 wera Duce tbo koe eee e nus. 18 
SHEE, BADE, C6030 5.07 Snails cc.cicie re sarieeerer 18 
MAIMNMOR a5 ccladac.cns ssh sau eh etG hear mentee ee 19 
PGR occas ssirnccces caats ahaa hansen 20 
Spores—Endospores, Arthrospores.......... 21 
Cell-unions—Zoogloeae, Chains, Filaments.. 22 
Fssvolition-fOrms .. ss5 soca. cede ae na ciseinaias 23 
General Comment). scccivccteacrieccoand ss 23 
PRYSOIOPY: 9:5 ceils oie oe lols s Seta alel Bane He swale 25 
MOUULY << 0)2 foc oricesteucieiiiins oh pr ecucree 26 
GLOW case cl sidiaccrereiins Gc creee cies Cees Se, waweorens 27 
Chemotropigty cis. «5.5200 tcsiccete sow oscsits en 27 
REACtOs 10, Stasis ss ncoe se 0 ote’ Actecssienwtass 27 
AC ONEMER BEMIS i 5 vcsice beads ciae's os se eeae wre 29 
Nutrient Gelatin och. Giccsesnseet cee ces 29 
Neen Agar o's cica:c 5c acs esses case 31 
SHULECHUEAT OILY oS coins a av a2 wale pice te steaernn s 36 
Solid Vegetable Substances.............. 30 
Plant Juices (with and without the addi- 
Hom OF \Watel). oiiisdteccae cdpccitess oe 41 
Martial MIMS 55. sees cealnuke rain cea 45 
Peeleirain os ena eee ees 45 
ME acts aloud neweceweeg esteyecenn aie ss 46 
Postings (MSC oo aa tesscceoheceasarns 48 
Rice: cooked st Mik... 6.5c.000 ss0s oases 48 
Loeffler’s Solidified Blood-serum........ 48 
Raegoalbumen= 35 ccs nose scndeces ta 4 48 
Meyavolleiccccss sss sidoesanincen tennis 49 
‘Synthetic Media and Other Special Media. 49 
Relation to Free Oxygen...............006- 51 
Surface and Deep Growths............... 51 
Fermentation-tubes ..........00s0s-ee00- 52 
Growth in Hydrogen, in Carbon Dioxide, 
in Vacuo, and in Nitrogen........... 54 
EUMNIUINOSIEY coc cmd ieee emma Tle oes cere 60 
The Organism—Continued. 
Physiology—Continued. 
Fermentation-products .............0000005 
Alkalies (Ammonia, Amins, etc.).......... 
Reducing Powers: ya isesascsenanne soe ane 
Hydrogen Sulphide. far aecahinccaneree see 
Mercaptan and Other Odors................ 
Indol, Phenol, Leucin, Tyrosin, etc......... 
Reduction of Nitrates, ete,!.. 5 33.225. .205eee 
Fixation of Free Nitrogen, etc............. 
Assimilation of Carbon Dioxide............ 
Pig mente oc 2 cesta chars ae cite sieeutah eretaetiae tee 
Crystals jsudsesedae tas sesinecsiues ahiesee ae 
Bi Zyil6S. vs leis ¢ ssisrersrore vies congas conaatn texte 
Sensitiveness to Plant Acids............... 
Sensitiveness to Alkalies.................65 
Effect of, Desiccation® « sisisresaaas-01ace nese 
Effect of Direct Sunlight.24s¢s49 oaceco2s 
Vitality on Various ‘Media................. 
Mixed Cultures and Mixed Infections...... 
Reaction to Antiseptics and Germicides..... 
Thermal Relations: Maximum, Minimum, 
and Optimum ‘Temperatures for 
Growth; Thermal Death-point; Effect 
OEP LCOZNGS ose sas cecnne ohiorswas eee 
Other *Host-plants: ca: s.53c00coiieesseaee se 
Pathogenic or Non-pathogenic to Animals... 
Beonomic, Aspectsenic garcia viscera eenepn nee 
TOSSOS. se Se aieeih een es a aeeee dy oacaa wa Veintiews 
Conditions favoring Spread of the Disease. ... 
Methods of Prevention «.0(:s:6.3. 0.06 S's sidaisiaietes' se 
General ‘Considerations: 09 :s:650d iiss oatiewste ss a 
Location: of ‘the laboratory sc. 2iseacc ie sceinnne 
Equipment of the Laboratory................- 
Care of the ‘Laboratory’. 60a csiisas sae se asa ncies 
Preparation and Care of Culture-media....... 
The Cleaning and Sterilization of Glassware 
and snstriutents’ ances te cece ears 
Making and Transference of Pure Cultures... 
The Final Disposal of Infectious Material.... 
Methods’ of Inoculationiis....i.e0c caste cscaaee 
The Keeping Of Records: siie.at:s cas cnc sas see 
The Making of Collections................08. 
Distilled Water sfc care acs toe s0a1 6 ds0re stelere efecera,eelo 
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