72 . BACTERIA IN RELATION TO PLANT DISEASES. 
better, on larger Petri dishes filled with pounded ice; otherwise, in case of 30 to 60 
minute exposures, the temperature may rise nearly or quite to that of the thermal 
death-point of the organism, and then we shall have the effect of heat complicating 
that of light. ‘To avoid errors it is always best to take one-half of each dish as a 
check (rather than the whole of a separate dish), and the rise of temperature should 
be carefully recorded. In some tests made by the writer in Washington in May the 
temperature of the plates exposed in the open air to the sun for 45 minutes (without 
ice) rose from 25° to 51° C. Figs. 61 and 62 show the effect of sunlight upon thin 
sowings of Bacillus amylovorus and Bacterium phaseoli in poured-plate (Petri-dish) 
cultures. 
VITALITY ON VARIOUS MEDIA. 
By this I mean the determination of the resistance of organisms to their own 
decomposition products. This varies greatly. Much may be learned by the study 
of old cultures. Do not discard test-tube cultures until after many weeks. Examine 
frequently. Make transfers from tubes which have been inoculated for a year or 
more. Determine whether this vitality is due to spores or persists in the ordinary 
vegetative rods. On what kinds of media does a particular organism live longest? 
Can length of life be increased by occasionally neutralizing decomposition products 
(acids) with sterile carbonate of lime? or by occasional additions of food? Some 
bacteria are veritable revelers in filth; others are extremely setisitive ; all are soon 
under abnormal conditions in our culture-tubes. 
Another way of keeping bacteria alive for a long time is by reducing their growth 
toaminimum. Stock-cultures, especially of perishable organisms, should, generally 
speaking, be kept in the ice-box at temperatures under 15°C. ‘This greatly reduces 
the always heavy burden of keeping alive cultures of organisms which are not 
in immediate demand for actual experiment. Some will also remain alive a long 
time when sealed airtight. Particular organisms may be kept a long time in par- 
ticular media, e. g., Bacterium vascularum in diluted peptonized cane-juice gelatin, 
Bact. Stewarté in milk, etc. Some organisms are quite resistant to their own 
decomposition products, e. g., Bacillus coli, Bact. pericarditidis. In the cool box 
B. coli will often live a year in agar stab cultures. 
MIXED CULTURES AND MIXED INFECTIONS. 
The behavior of mixed cultures and mixed infections may be tested in various 
fluids, making poured plates from time to time; in tubes of agar, potato, and other 
solid media ; in crossed streaks on agar or gelatin plates; and in the plants themselves. 
When two bacteria, or a bacterium and a fungus, are sown together in a culture- 
medium, there may be (1) antagonism, with the crowding out of one species ; (2) a 
more or less complete indifference, both organisms growing well; or (3) a distinctly 
favorable effect, 7. ¢.,a marked increase in growth or in pathogenic effect due to 
the presence of the second organism. ‘The antagonism may result in the prompt 
destruction of one of the organisms, or only in a retardation or inhibition which 
finally disappears after the first organism has made its growth and subsided. In 
some cases the favorable effect of one organism upon another is due to the fact that 
it prepares food for it out of an unfavorable substratum, e. g., maltose from starch. 
