184 BACTERIA IN RELATION TO PLANT DISEASES. 
Of the inoculated trout one died at the end of 15 days. The rest (with one exception) 
died in from 30 to 40 days from the time of inoculation. The relative rate of death of 
inoculated and check trout is shown in the following table: 
Taste SHowinc Dare or Deatu AND Symptoms OssERVED IN TROUT. 
Inoculated Trout. Check Trout. 
\ - j —s = ee 7 
| Date. | No. Remarks. | Remarks. 
j lin han ee acai = = rs 
1908. | | 
Apr. 4 Vii Btw ah oh sciatic de beets pobacas wae mene ets GERe aaa eas ae eaie mete From Mar. 20 to Apr. 29, no deaths among 
Apr. 22 | II, 1 Congestion in region of anus, base of tongue, liver, heart. | the roo check fish. 
Apr. 23. =~*VI, 1 Eye-socket infl d. Liver di d During this period 10 deaths among the 18 
| | I,1 Hard yellowish rough tumor on tongue (the only hard tumor). Eye- inoculated fish. _ 
| | socket inflamed. ei On Mar. 25, 3 showing throat tumors were 
Apr. 25 | XIV, 1 Walls of lower intestine inflamed, ulcers on inner belly wall and separated from the rest of the checks. 
} | externally at root of pectoral fin and on outside below anal fin. 
Throat sound. 
II,1 Angmic. Ulcerous tongue and inner belly wall. 
Apr. 28 Iv, 1\A i Eye-sockets infl d. Vicinity of thyroid inflamed and 
slightly swollen. 
V, 1 Eye-socket inflamed. Liver white-mottled. Spots on gills and 
| region of the thyroid inflamed. 
Apr. 29 IV, 1 Stomach and intestines much inflamed. White patch on liver. 
Back part of throat inflamed, i. ¢., below the tongue. Inflammation 
in throat where needle-pricks ended; None outside. 
XIV,1 Marked infl ion of abdominal wall where needle entered. Needle 
wound healed externally. Inflamed spot at base of tongue. Lower 
intestine much inflamed. 
Apr. 30 IV, 1 Bloody patches on lower intestine. Ulcerontail. _Apr.30 1. The first check to die. Tumor 
in tl t. 
May 1 III, 1 Several small ulcers on surface. Throat sound, liver diseased, in- 
flamed patches on inner wall of abdomen. | 
May 2 I,1 Small abrasion on surface, also a Saprolegnia patch (3 sq. in.). Gills, 
stomach and intestine congested. Eye-socket badly inflamed. 
May 4 XIII, 1 Eye on inoculated side is white-clouded, swollen inflamed tissue at | 
base of eyes, especially on inoculated side. Marked congestion of | 
| — a wall of abdomen in lower part; tongue somewhat swollen | 
| an uy | 
| May 5 V, 1 | Small sore spot at roots of the tail. Throat sound. Membrane cover- 
ing the intestine and eye-socket highly inflamed. Also inflamed 
places on the inner lower belly wall. 
May 6. Two, with cancerous growths in 
throat. 
May 7 III, 1 Notumor on the adipose (where inoculated). Base of tongue swollen 
| and inflamed outside and inside. 
| | May8. 1. Cancerous thyroid. 
| | Mayg. 1. Mouth sore. 
May 11 XIII, 1 Throat and gills sound, eye-socket inflamed, viscera swollen and May tr. 7. Two have sores in the mouth. 
inflamed. Inner belly wall badly inflamed. 
May 14. 3. Oneanemicand with an ulcer 
= the gills. Weather hot for last three 
ays. 
Mayts5. 1. Cancer in thyroid region. 
: | May 16. 4. One hasa cancerous throat. 
| May 19 XIV One alive on this date when experiment was abandoned. _ May rg. 7. Not dissected. 
Since the above paragraphs were written the most hopeful portions of the inoculated 
trout have been infiltrated, sectioned, stained, and studied, with the following results 
(the figures in parenthesis refer to the preceding table). 
519 (April 28, V). Ulcer on inner wall of abdomen. Proliferations too regular for sarcoma. 
Probably not malignant. 
520 (April 25, XIV). Hypodermic. Ulceron inner belly wall nearentrance of needle. Sarcoma (?). 
Very suspicious. 
546 (April 23, IV). Proliferations—not malignant. 
549 (April 25,11). Tumor on tongue: Adenocarcinoma. 
621 a (April 23, I). Portion of tongue. Probably not malignant. Some of the cartilage has 
an abnormally large number of cells in it (chondroma?) . 
ae 622 (April 12, II). Inflamed part of inner wall of abdomen. Proliferations but nothing 
efinite. 
642 (April 19, XIV). Sections of small swellings on inner belly wall where inoculated. May 
be accounted for as simple inflammation. Later: There are giant cells in it. 
625 a (May 4, XIII). Hypodermic, eye-socket. Ulcer at base of eye. Typical giant cells, 
but possibly foreign body giant cells. 
DO PLANTS HARBOR ANIMAL PARASITES? 
In a paper published in 1889 in the Comptes Rendus of the French Academy, Dr. 
Domingos Freire undertakes to show that roses and various other common flowers harbor 
bacteria, some of which are pathogenic to man. All this paper really proves is that which 
a 
