WILT OF CUCURBITS. 241 
bundle contained bacteria (small bacilli) in large numbers. Higher up one or two vessels contained 
bacilli of much larger size and in smaller numbers. Some of the vessels also contained a branching 
mycelium suggestive of Fusarium. The pot stood on a bench where watermelon wilt experiments 
were carried on previously. 
(80.) Winter Squash var. Hubbard. The blade of a leaf 1.25 inches broad was pricked many 
times at the apex. Two months after inoculation the vine was brought into the laboratory. All but 
a few upper leaves were shriveled. The stem was still green and turgid. The latter was examined 
in three places for bacteria but none were found. 
(8r.) Winter Squash var. Hubbard. A leaf 4 inches broad was pricked many times at the apex. 
No result. Owing to crowding in a small pot only the upper leaves were alive on January 28, and 
these were dwarfed. The green stem was examined for presence of bacteria, but none were found. 
(82.) Winter Squash var. Hubbard. A leaf 4 inches broad was pricked many times at the apex. 
February 28 the vine was wholly dried out. No bacteria were present in the vessels or parenchyma 
of the stem. 
Remarks.—None of the potatoes or tomatoes took the disease and only two of the 
muskmelons. The squashes proved very resistant. They were in 4-inch pots and grew 
well for some time after inoculation. No. 79 was not affected at first but seemed to be 
affected after a long time. On December 20 the squashes were still in 4-inch pots and 
growing satisfactorily. 
The early maturity of the potatoes undoubtedly resulted from keeping the plants too 
long in small pots. All the potatoes in the hothouse behaved in the manner described 
irrespective of whether they were inoculated or not. The tubers were planted in November 
and the shriveling began in December and on January 14 was apparent on all but two plants. 
Several examinations showed no bacteria or fungi in the tissues and the plants had not 
suffered from aphides or red spiders, nor had they been neglected or frosted. They were 
in 4-inch pots for about a month when they were transferred to 6-inch pots (about January 
4) to see if this would help them to recover. It did not, however. When repotted most of 
the vines were over 2 feet high. These facts favor the supposition that the yellowing and 
shriveling was a natural one, occurring after the plants had performed their life-work, which 
was hastened by the small size of the pots. 
From this experiment it was evident that the disease could be produced in the musk- 
melon with bacilli taken from the cucumber, but not with certainty in squashes. 
The temperature in the hothouse the first two weeks after inoculation (December 6 to 
20) varied from 60° to 90° F. 
INOCULATIONS OF DECEMBER 10, 1894. 
Fifteen plants including 3 hyacinths (Hyacinthus orientalis), 2 Hubbard squashes 
(Cucurbita sp.), 2 summer crookneck squashes (Cucurbita sp.), 1 potato, 2 cucumbers, 4 
tomatoes, and 1 cantaloupe, were inoculated in the hothouse, with a white wet-shining, 
sticky, motile bacillus (cucumber-strain) growing on a potato-cylinder (pure culture No. 5, 
December 6, from potato-broth No. 2, December 3). The greatest pains was taken to do 
the work thoroughly. After each plant was thoroughly pricked I went back over the 
bench and pricked them again. Much material was used in pricking which was done in the 
afternoon. More than half the bacteria in this culture were motile. The temperature of 
the hothouse from December 6 to 20 varied from 60° to go° F. 
(83a, 84, 85.) Hyacinth. These plants.had been potted 3 days at the time of inoculation and 
the green bud had pushed up 1 to 2 cm. In each 15 or 20 needle pricks were made into the bud, 
some of them deep. Up to the eighteenth day there was no sign of the blight. 
(86.) Hubbard Squash. At the time of inoculation this vine was about 2 feet high and had 
6 good leaves. It was very thrifty. About 40 pricks were made on the apex of the blade of the third 
leaf which was about 5 inches broad. 
Up to February 27 (79 days) the vine showed no trace of. the wilt. The stem was then examined 
in several places but the vessels and parenchyma were free from the bacteria. 
