252 BACTERIA IN RELATION TO PLANT DISEASES. 
(156.) Pumpkin var. Nantucket Sugar (Cucurbita sp.). Many pricks were made on various 
parts of a leaf-blade about 2.75 inches broad. 
> There was no result from the inoculation. 
(157.) Pumpkin var. Nantucket Sugar. This vine which was growing in the same pot as the 
preceding was pricked many times on the middle and apical portion of a leaf-blade about 3.5 inches 
broad. 
There was no result from the inoculation. 
(158.) Pumpkin var. Nantucket Sugar. This vine was in the pot with 156 and 157. Many 
pricks were niade on the middle and apical part of a blade about 2.5 inches broad. 
There was no result from the inoculation. 
(159.) Pumpkin var. Nantucket Sugar. Many pricks were made on the apical part of a leaf- 
blade about 2 inches broad. 
There was no result from the inoculation. 
(160.) Pumpkin var. Nantucket Sugar. This was growing in the pot with the preceding. Many 
pricks were made on the apical portion of 
a leaf-blade about 2 inches broad. 
No disease resulted. 
(161.) Pumpkin var. Nantucket 
Sugar. Many pricks were made on the 
apical portion of a leaf-blade about 3 
inches broad. 
No result. 
(162.) Pumpkin var. Nantucket 
Sugar. This vine was growing in the pot 
with 161. Many pricks were made on 
one side of a leaf-blade about 4 inches 
broad. 
No result. 
(163.) Watermelon (Citrullus vul- 
garis). This vine, which was planted 
March 12, was pricked many times on a 
side lobe of a leaf-blade about 1.75 inches 
broad. 
There was no result from the inocula- 
tion. 
(164.) Watermelon. ‘This vine was 
about a month old. Many pricks were 
made on the apical part of a leaf-blade 
about 1 inch broad. 
No resuJt. 
(165.) Watermelon. This vine was 
the same age as the preceding. Many pricks were made on the middle lobe on one side of a leaf- 
blade about 1.25 inches in diameter. 
No result. 
(166.) Watermelon. This was growing in the pot with the preceding. Many pricks were made 
on the middle and basal lobes on one side of a leaf-blade about 2 inches broad. 
No result. 
(167.) Muskmelon var. Shumway’s Giant. About 20 pricks were made in the center of a 
leaf-blade over 2 inches broad. ‘The pricks were in a space not over 5 mm. in diameter, and to each 
side of the midrib. The fifth day (9 a. m.) the first trace of wilt appeared. It extended in a narrow 
line along the midrib from the pricked area to the tip. It was most noticeable at the extreme tip. 
At 2 p. m. the apical one-sixth of the leaf-blade had wilted in a V-shaped area from the pricked part 
outward. ‘The wilt did not yet extend downward beyond the pricked area more than 1.5mm. The 
next afternoon about one-third of the pricked leaf-blade (apical part) had wilted and changed color. 
The seventh day there was no change. The next morning the blade of the first leaf down and of the 
first leaf up had wilted. The petioles were rigid. About two-thirds of the blade of the pricked leaf 
was flabby. The ninth day the cotyledon under the pricked leaf had wilted. The opposite one was 
green and turgid. The second leaf up was flabby. The leaves of the bud were still normal although 
*Fic. 69.—Cross-section of middle of a muskmelon petiole,showing a bundle disorganized by Bacillus tracheiphilus 
with the formation of a large cavity. From inoculated plant No. 150. Drawn from a paraffin infiltrated stained 
section. Slide No. 208 A 9. 
