WILT OF CUCURBITS. 273 
but 4 days later there was no wilt. On July 14 the pricked leaf was shriveled but the rest of the vine 
was normal. : ' ; : 
(303.) Cucurbita digitata. Wilt began on the fifth or sixth day in the middle of the pricked part. 
‘The seventh day in the central part of the pricked area the tissue for 0.7 sq. cm. was dead and yellow- 
white. Outside of this was a narrow border of freshly wilting tissue, but five-sixths of the leaf was 
still normal. ‘T'wo days later the whole of the pricked blade and the upper two-thirds of the petiole 
had shriveled. The eleventh day the second leaf was beginning’ to yellow and droop. On July 14 
the pricked leaf and the first leaf up had shriveled. The rest of the vine was normal. 
(304.) Trichosanthes cucumeroides (from Agr. college in Japan). The seventh day the tip of the 
pricked leaf was wilting but 2 days later most of the pricked leaf was normal and the wilt seemed 
to be dying out. The eleventh day a fresh part of the leaf had begun to wilt. On July 14 the 
whole of the pricked leaf had shriveled. The rest of the vine was normal. 
(305.) Trichosanthes cucumeroides. The pricked leaf showed no signs up to June 25. The 
eleventh day about one-third of the apical (pricked) portion of the inoculated leaf-blade had wilted 
but was not yet dry. On July 14 the pricked leaf had shriveled, but the rest of the vine was normal 
and was growing rapidly. : ; 
(306.) Trichosanthes cucumeroides. There was no result from the inoculation other than local 
injury which did not appear until after June 27 (eleventh day). On July 14 the pricked leaf had 
dry-shriveled but the remainder of 
the vine was normal and growing 
rapidly. 
(307.) Passiflora incarnata. 
The seventh day a whitish callous 
had formed around each of the 
pricks and there was no wilt. Up 
to July 14 there had been no result 
even in the pricked leaf. 
(308.) Passiflora incarnata. 
Like the preceding. No result. 
There was no wilt or change of 
color even in the pricked leaf. 
(309.) Cucumis melo var. 
dudaim (ripe fruit yellow and like 
a’ small round gourd; delightful 
odor; taste like muskmelon). The 
inoculation was made in an old 
leaf. ‘The seventh day there was a 
distinct wilt in and around the 
pricked area. This had begun ina 
small way the preceding day. Two Koo 
days later about one-fourth of the ama 
pricked leaf-blade was wilted. The 
blade of this leaf measured 3.5 X3.5 
inches. On the eleventh day the 
whole of the pricked leaf-blade 
was dry-shriveled. On July 14 the 
whole plant was dry-shriveled. 
(310.) Citrullus vulgaris. The Fig. 78.* 
seventh day there was no wilt and 
it looked as if a cork-layer had formed around the pricks, at least there was a narrow yellow rim 
around each prick (estimated 0.1 mm. wide). The plant was examined June 25, 27, July 3 and 14. 
There was no result even in the pricked leaf. This leaf had received 67 pricks. 
(311.) Echinocystis lobata. Wilt appeared the sixth day in the pricked portion of the inoculated 
leaf. The seventh day nearly the whole of the pricked leaf had wilted and 2 days later the whole 
pricked blade including the tip of the petiole had shriveled. This leaf was separated from the one 
above by an internode of 4 inches and that leaf was still normal. The first leaf below was 4 inches 
down and that too was unaffected by the wilt. The eleventh day the plant had developed as fine 
a case of the bacterial wilt as could be desired. The pricked leaf had entirely shriveled, including 
*Fic. 78.—Cross-section of petiole of Cucurbita foetidissima, showing a bundle occupied by Bacillus tracheiphilus 
as result of a pure culture inoculation on lamina of leaf. For orientation see fig. 77, which was made, however, 
from another section. Drawn from slide 254 B x, with the Abbe camera. Plant No. 273. 
