WILT OF CUCURBITS. 283 
were no signs on any of the plants until after July 30. The Mexican plant is Dr. Edward 
Palmer’s No. 1801a, and was grown from seeds of his collecting. 
(430.) Cucumber. Up to August 2 (seventh day) there was no result from the inoculation. 
(431.) Cucumber. The fifth day there was a distinct wilt of several square centimeters around 
the pricks on each leaf. 
(432.) Cucumber. The fifth day there was a distinct wilt of one square centimeter in the pricked 
area on one leaf while the whole blade of the other leaf was wilted and collapsing. 
(433.) Cucumber. The fifth day there were several square centimeters of wilt in the pricked 
area on both leaves. 
(434.) Cucurbitaceous plant (collected in Mexico). No result by the sixth day. No further 
record. 
(435.) Duplicate of 434. The sixth day both leaves were normal. No further record. 
Remarks.—Three out of the four cucumbers contracted the disease promptly. The 
ill-scented Mexican plant bore yellow flowers; long, warty fruits, and leaves suggestive 
of Momordica. 
INOCULATIONS OF AUGUST 21, 1897. 
A series of inoculations was made on watermelon vines (Citrullus vulgaris) in a garden 
at Hubbardston, Michigan. Thebacteria were taken froma potato-culture of B. tracheiphilus 
(tube 2, July 23) which had been re-inoculated July 26 with 0.2 cc. out of tube 1, July 19. 
This potato-culture was very sticky and in fine condition on August 12 when it was exhibited 
at the Detroit meeting of the American Association for the Advancement of Science, but at 
the time of the inoculations it had been considerably exposed to the light and was past its 
prime, although I believed it to be alive. It was not, however, tested under the microscope 
or by transfer to other media as it would have been had I had laboratory facilities. The 
inoculations were made in the ordinary way, i. e., by means of a dozen or two needle-punc- 
tures on the leaf-blade and within an area of 2 or 3sq.cm. The extreme upper part of the 
potato bore the stickiest slime and this was used for most of the inoculations, but some were 
made in the following way: Numerous punctures were made and then the tube was tilted 
until the cotton plug was wetted. This plug was then taken and mopped over the pricked 
area. The fluid in the bottom of this tube was very milky. The inoculations were made at 
sunset so as to avoid the immediate evil effect of light. As a check on the virulence of the 
culture eight muskmelon leaves belonging to five plants were inoculated in the same way. 
On four leaves the bacteria were pricked in; on the others they were mopped in after the 
pricks were made. - 
(436-439.) Watermelons. Sixteen healthy leaves belonging to four vines were inoculated, 
eight in the ordinary way, four by the second method described. 
There was no result. 
(440-444.) Muskmelons. Eight leaves belonging to five plants were inoculated, four by the 
second method. 
The inoculations failed. 
Remarks.—The observations were continued until September 12. The weather was 
very dry. Probably the potato culture was dead. It will be remembered that it was 26 
days old and that it had been very copiously inoculated on the start. 
INOCULATIONS OF JULY 11, 1898. 
A series of inoculations was made on the wild bur cucumber (Sicyos angulatus) growing 
in a garden at Anacostia, D.C. The plants were large and covered the ground, the leaves 
being 4 to 8 inches broad, and all were perfectly healthy. All of the inoculations were made 
by pricking in the bacteria (cucumber-strain) with a sharp steel needle. 
(445-456.) About a dozen leaves belonging to several different plants were inoculated in the 
blades from a pure agar-culture (tube 3, June 30, re-inoculated July 7.) The seventh day there were 
seven beautifully typical cases in as many of the pricked leaves. These were in all stages from one 
just beginning to change color and wilt around the punctures to one wholly collapsed. There was 
