1^6 TUBERCULIN TEST 



subsequent test previous to selling showed no reaction. A doubtful, 

 but possible, case of recovery. 



Bessie, Ayrshire cow, five years old. At a test made December 5 

 and 6, 1895, previous to transferring the herd to the new barn (see 

 Bulletin 31). Bessie showed a reaction of 4.6 degrees; she was con- 

 demned and placed in quarantine. December 19 and 20, 1895, she 

 was tested again and showed a reaction of 2 degrees. In September, 

 1896, she was tested a third time but failed to show a reaction. She 

 was killed, however, and a careful examination at the autopsy 

 showed no traces of the disease. A probable case of recovery. 



^Experiments ivith the milk of the condemned cows : — An 

 effort was made to determine whether bacilli were present in 

 the milk of the condemned cows by microscopical examina- 

 tion, by inoculation of guinea-pigs, and by feeding calves with 

 it. 



INOCULATION OF GUINEA-PIGS WITH MILK OF TUBERCULOUS 



COWS 



December 29, 1894. Began inoculation of guinea-pigs 

 with milk from the tuberculous cows. The following was the 

 general method of procedure : The udder and hind quarters 

 of the cow were cleaned with a brush, when necessary, and 

 then thoroughly moistened with water by means of a sponge 

 to prevent any falling of dust from the cow into the milk. The 

 milk was then drawn into sterilized flasks ; from these flasks 

 the milk used for inoculation was drawn. 



The hair on the belly of the guinea-pig, at the point chosen 

 for inoculation, was clipped oft' with scissors and the surface 

 of the skin was scrubbed with dilute carbolic acid. The milk 

 was then injected into the peritoneal cavity by means of a ster- 

 ilized Koch syringe. 



December 29. Inoculated a male guinea-pig (No. 1) with 1 cubic 

 centimetre of milk from Durham No. 6. The milk used was the first 

 drawn from the udder at night. 



January 2, 1895. Inoculated a male guinea-pig (No 2) with 1 cubic 

 centimetre of morning's milk from Durham No. 6. The milk was a 

 mixture of the first milk and the strippings. 



January 5. Inoculated a male guinea-pig (No. 3) with 2 cubic cen- 

 timetres of night's milk from Pilot's Lily. Tbe injection was partly 

 subcutaneous. The sample used was from the first milkings. 



