APPENDIX C. 



tion ; the fluid was found to be devoid of all odour, it had a 

 slightly bitter taste, and its re-action was very faintly acid. 

 On microscopical examination no living things were found ; 

 there were no Bacteria, no Vibriones, and no Torulce, only 

 some mere granules, a small amount of amorphous matter, 

 and a few fibres *. 



No. XXXIV. Turnip Infusion Neutralized by Am- 

 monic Carbonate in forty-eight hours showed a slight tur- 

 bidity, which slowly increased during the next two days. In 

 two days more the turbidity was very great, and there was 

 also a considerable amount of sediment. The fluid was then 

 examined microscopically, and found to contain myriads of 

 large but very languid Bacteria. 



e. Fluid (in vacuo) in a Flask which had been Sealed during 

 Ebullition. 



No. XXXV. Healthy Urine underwent no apparent 

 change for the first twelve days, then (the bulk of the fluid 

 still remaining clear and bright) small greyish-white flocculi 

 began to collect at the bottom of th^ flask, which very 

 slowly increased in quantity during the succeeding twelve 

 days. At the expiration of this time the flocculi were pretty 

 numerous, though the fluid was otherwise bright. The 



turbid in thirty-six hours, and was then found to contain multitudes of 

 Bacteria ; and the characteristic odour of the turnip infusion was still 

 appreciable. 



1 The results of this experiment are most interesting, especially if 

 compared with that which takes place when some of the same fluid is 

 neutralized by ammonic carbonate (No. XXXIV.), or when a similar 

 fluid (as in No. XXX.) is contained in a flask sealed during the ebulli- 

 tion of the fluid, or with what occurred in Nos. XIII. and XXXII. 

 In the present case the second boiling seems to have destroyed what 

 small amount of fermentability there was still remaining in the solu- 

 tion ; but in No. IX. fermentation did take place after the second 

 boiling though it occurred only under the influence of diminished 

 pressure and a higher temperature. 



