xliv THE BEGINNINGS OF LIPE. 



from deposit until the twelfth day, when it was examined 

 microscopically. No organisms of any kind could be de- 

 tected. 



No. XLIII. Hay Infusion + ^V part of Carbolic 

 Acid, showed no apparent change 1 for the first five days, 

 though, on the sixth day, a slight deposit was noticed at the 

 bottom of the flask. The deposit had increased, and was well 

 marked by the twelfth day, when, on microscopical examina- 

 tion, there were found, amongst the granular flakes of the 

 deposit, Torulce of several varieties of size and shape. Many 

 were spherical, others ovoid, or having an elongated oat-like 

 shape ; some were of the ordinary colour, and others were 

 brownish in tint. The variety was most striking. No Bac- 

 teria were seen, though there were multitudes of active parti- 

 cles which seemed to differ from the minute spherules of 

 undissolved carbolic acid. 



Fluids (in vacuo) boiled for five minutes, and Flasks Sealed 

 during Ebullition. 



No. XLIV. Turnip Infusion, in seventy-two hours, 

 showed a slight turbidity, which gradually increased. On the 

 eighth day there was a considerable quantity of flake-like 

 sediment, and some amount of general turbidity. On the 

 thirteenth day the vacuum was found to be still partly pre- 

 served. When the flask was opened the fluid was perceived 

 to have a foetid odour, and an acid re-action ; and, on micro- 

 scopical examination, multitudes of Bacteria and Vibriones 

 were seen. In the flake-like aggregations (made up almost 

 wholly of these organisms) there were also a number of 

 large thick-walled spores ; some already formed, and others 

 in process of formation. 



1 The alteration in colour was less marked than in the similar mix- 

 ture which had not been boiled, though the turbidity was just as 

 obvious. 



