APPENDIX A 505 



by alcohol. It may be prepared from the substance as supplied 

 by the dealers, or by extraction of seedlings of Lupinus luteus 

 with water, and evaporation (see p. 228). 



Asphalte is used for sealing up slides in which glycerine has 

 been used as a mounting medium (see p. 52) : it is liable to 

 become very brittle after a time, and to prevent the cement 

 breaking away, it may with advantage be covered with a layer of 

 gold-size. It may be obtained ready for use from the dealers. 



Benzol, used as a solvent for various substances, e.g. the 

 coagulum of latex, eerie acid (p. 40), &c. 



Brunswick Black may be bought ready prepared from dealers 

 in microscopic requisites : it is used for sealing up slides (p. 52). 



Calcium Chloride has been recommended for the preparation 

 of sections of growing points : it is to be used as directed on p. 50. 



Callus-reagent of Russow is prepared by mixing equal 

 volumes of chlor-zinc-iodine, and of the solution of iodine in 

 potassium iodide : it stains the callus of sieve-tubes a deep brown 

 (p. 115). 



Canada Balsam is to be used dissolved in benzol and in such 

 quantity that it shall have the consistency of a syrup. It is used as 

 a mounting medium for sections previously treated with alcohol, 

 and then, with either oil of cloves, turpentine and creosote, or 

 cajeput oil (see p. 52) ; it is also used for sealing up slides. 



Cane-Sugar, The concentrated" solution in water is sometimes 

 used, together with strong sulphuric acid, as a test for proteids. 

 A dilute solution (1 per cent., or more) is useful for mounting 

 living cells for observation under the microscope (see p. 202). 



Carbolic Acid (see Phenol). 



Carmine. The two best preparations of carmine are those of 

 Beale and Thiersch. 



1. Beale's Carmine. To prepare this, 0'6 gramme of carmine is 

 dissolved in 2 c.c. of boiling solution of ammonia ; the solution 

 must then stand for an hour or so to cool, and to allow of the 

 escape of the superfluous ammonia ; to the solution are added 

 60 c.c. of distilled water, 60 grammes of glycerine, and 15 grammes 

 of absolute alcohol. The mixture must be allowed to stand for 

 some time ; it is then to be filtered. 



2. Thiersch's Carmine. 4 grammes of borax are dissolved in 



