APPENDIX. 259 



5. Hsematoxylin, Solution of. 



a. Prepare a saturated solution of crystallized calcic 

 chloride in 70 per cent, alcohol; then add alum to 

 saturation. 



b. Prepare a saturated solution of alum in 70 per cent, 

 alcohol. Add 1 volume of a to 8 of b. 



c. To the mixture of a and b add a few drops of a 

 saturated solution of pure hsematoxylin in absolute 

 alcohol. Filter. 



6. Iodine, Solution of. 



Prepare a saturated solution of potassic iodide in distilled 

 water ; saturate this solution with iodine. Filter. Dilute 

 to a brown sherry colour. 



7. Magenta, Solution of. 



Dissolve 1 decigr. of crystallized magenta (roseine) in 160 

 cubic centimetres of distilled water : add 1 cub. cent, of 

 absolute alcohol. Keep in a well-closed bottle. 



8. Mayer's Solution. 



See note, p. 8. 



9. Miiller's Solution. 



Bichromate of potash 25 grammes. 



Sodic sulphate 10 grammes. 



Distilled water 1 litre. 



10. Osmic Acid, Solution of. 



Best bought ready made in the form of 1 per cent, solution. 



11. Paraffin. 



Melt together one part of solid paraffin (paraffin candles 

 will do), one part of paraffin oil and one part of pig's lard. 

 A mixture in the above proportions gives, when it has 

 cooled, a mass of the most generally useful consistency. 



172 



