594 LABORATORY EXERCISES 



Hygienische Rundschau, Bd. iv, 1894, p. 769. 

 Fermi's Culture Fluid. 



Distilled water 1000. o c.c. 



Magnesium sulphate o 2 gr. 



Acid potassium phosphate i . o 



Ammonium phosphate 10. o 



Glycerin 450 



This may be added to agar in place of peptonized beef-broth (De jSchweinitz) 

 or to silicate jelly in which case the volume of water must be reduced. 

 Knop's Solution. 



Calcium nitrate (Ca(No3)2, gram i .00 gr. 



Calcium chloride (KCl), gram o. 25 



Magnesium sulphate (MgS04), gram o. 25 



Acid potassium phosphate (KH2PO4), gram o 25 



Distilled water, c.c 1000.00 c.c. 



Mdisdi's Culture Medium {for luminous bacteria). 



Water 1000.00 c.c. 



Gelatin 100.00 gr. 



Sugar 20 . 00 



Pepton 10.00 



Dipotassium phosphate o. 25 



Magnesium sulphate o. 25 



Enough sodium hydroxide is added to render the medium fully alkaline. On 

 this substratum, the bacteria grow feebly and are not luminous until sodium 

 chloride, or some equivalent substance, is added (usually 3^ per cent.). Then they 

 grow well and become luminous. 



Leherle-Will Culture Medium {for Yeasts). — See KtJSTER, Ernst: Kultur der 

 Mikroorganismen, p. 143. 



CaHPOi, gram o. 50 



K2HPO4, grams 4-55 



MgSOi, grams 2 . 10 



Pepton, grams 20 . 00 



Water, liter i . 00 



Hansen's Culture Media jor Yeasts. 



Per cent. Per cent. 



Pepton I Pepton i 



Dextrose 5 Maltose 5 



Potassium phosphate 0.3 Potassium phosphate 0.3 



Magnesium sulphate 0.2 Magnesium sulphate 0.5 



