LABORATORY AND TEACHING METHODS 633 



LESSON 28 



The detailed investigation of the bacteria and other fungous organisms, as out- 

 lined below, can be undertaken only after the student has become acquainted with 

 the cultural methods given in another section of this handbook, but the table adopted 

 by the Society of American Bacteriologists is given below, because it fits into the 

 general discussion and study of the classification previously given. 



I. MORPHOLOGY. 



1. Vegetative Cells. — Medium used 



temp , age , days 



Form, round, short rods, long rods, short chains, long chains, filaments, commas, 

 short spirals, long spirals, Clostridium, cuncate, clavate, curved. 



Limits of size 



Size of majority 



Ends, rounded, truncate, concave. 



I Orientation (grouping) 



Agar I Chains (number of elements) 



hanging block I Short chains, long chains. 



[ Orientation of chains, parallel, irregular. 



2. Sporangia. — Medium used 



temp 



Form, elliptic, short rods, spindled, clavate, drum-sticks. 



Limits of size 



Size of majority 



Location of endospores, central, polar. 



3. Endospores. — Form, round, elliptic, elongated. 



Limits of size 



Size of majority 



Wall, thick, chin. 



Sporangium wall, adherent, non-adherent. 

 Germination, equatorial, oblique, polar, bipolar. 



4. Flagella. — No Attachment, polar, bipolar perilrichiate. 



How stained 



5. Capsules. — Present on 



6. ZOOGLCEA, PSEUDOZOOGLCEA. 



7. Involution Forms. — On in days at .°C. 



8. Staining Reactions. — i : 10 watery fuchsin, gentian violet, carbol fuchsin 

 Loeffler's alkaline methylene-blue. 



Special stains 



Gram Glycogen 



Fat Acid-fast 



Neisser. 



IL CULTURAL FEATURES 



I, 2, 3. Agar Stroke, Potato, Loeffler's Blood-serum. — 

 Growth, invisible, scanty, moderate, abundant. 



