LABORATORY AND TEACHING METHODS 



637 



8. Vitality on Culture Media. — Brief, moderate, long. 



9. Temperature Relations. — Thermal death point (ten minutes exposure in 

 nutrient broth when this is adapted to growth of organism) °C. 



10. Killed readily by drying, resistant to drying. 



11. Per cent, killed by freezing (salt and crushed ice or liquid air). 



12. Sunlight. — Exposure on ice in thinly sown agar plates; one-half plate cov- 

 ered (time fifteen minutes), sensitive, non-sensitive. 



Per cent, killed 



13. Acids produced 



14. Alkalis produced 



15. Alcohols 



16. Ferments. — Pepsin, trypsin, diastase, invertase, pectasc, cytase, tyrosinase, 

 oxidase, peroxidase, lipase, catalase, glucase, galactase, lab, etc. 



17. Crystals formed 



18. Effects of Germicides 



IV. PATHOGENICITY. 



1. Pathogenic to Animals. — Insects, crustaceans, fishes, reptiles, birds, mice, 

 rats, guinea pigs, rabbits, dogs, cats, sheep, goats, cattle, horses, monkeys, man. 



2. Pathogenic to Plants. — 



3. Toxins. — Soluble, endotoxins. 



4. Non-toxin forming 



5. Immunity (bactericidal)." 



6. Immunity (non-bactericidal) 



7. Loss of Virulence on Culture Media. 

 in months. 



-Prompt, gradual, not observed 



