APPENDIX. 



291 



Lung 



Tissvie or Organ. 



Mammary gland 

 Marrow of bone 

 Muscular tissue, striated . 



,, non-striated 



Nerve .... 

 (Esophagus 



Ovary .... 

 Pancreas .... 

 Retina .... 

 Salivary glands . 

 Sclerotic and cornea . 

 Skin ..... 

 Spinal cord 



Spleen .... 

 Stomach 



Suprarenal capsule . 



Tendon and ligament 



Testis 



Thymus gland . 



Thyroid gland . 



Tongue 



Tonsils 



Trachea 



Ureter 



Uterus 



Hardening Fluid. 



Distend with chromic acid or with 

 alcohol and chromic acid. 



Alcohol. 



Alcohol. 



Alcohol. 



Chromic acid. 



Picric acid or osmic acid. 



Distend with chromic acid. 



Chromic acid. 

 Alcohol. 

 Muller's fluid. 



Alcohol. 



Alcohol or Muller's fluid. 



Alcohol. 



Mtillers fluid. 



Muller's fluid, picric acid, or alcohol. 



Distend with chromic acid, or with 

 alcohol and chromic acid, or with 

 picric acid. 

 Alcohol. 

 Alcohol. 

 Alcohol. 

 Alcohol. 

 Alcohol. 



Muller's fluid or alcohol. 

 Alcohol. 



Chromic acid and alcohol. 

 Chromic acid and alcohol. 

 Chromic acid and alcohol. 



Tissues to be hardened in alcohol should usually be placed at once in strong 

 methylated spirit, or, better, in absolute alcohol. They are ready for cutting 

 as soon as they are dehydrated ; as a rule they may be left indefinitely in 

 alcohol without deterioration. Organs which contain much fibrous tissue, 

 such as the skin and tendons, should not go into strong alcohol, but should 

 be hardened in about 70 per cent, strength ; otherwise they become too hard 

 to cut. 



For tissues that are to be hardened in chromic acid an immersion of from 

 7 to 14 days is generally necessary ; they may then be placed in alcohol for 

 preservation and to complete the process of hardening. The spirit should be 

 changed once or twice. 



Organs placed in bichromate of potash or Miiller's fluid are ready for 

 section in a fortnight or three weeks ; they may, however, be left for a much 

 longer time in those fluids without deterioration. With picric acid the 

 hardening process is generally complete in 2 or 3 days ; the organs may 

 then be transferred to spirit, which ought to be frequently changed. 



