802 Illinois State Laboratory .of Natural History. 



not flagellate. Mounted slides show numerous spherical or 

 slightly quadrate micrococci, with many single and double ovals. 

 The spherical form is .75 ^ to 1 ^ in diameter, some of the 

 single ovals attaining a length of 1.5 /". The usual length of 

 the latter is, however, about 1.25 /*. 



Another larva of this lot died during the night of the 

 3d September, and was examined on the following morning. 

 Its intestinal contents were brown and nearly solid, requiring 

 to be moistened for examination. They were noted as "full of 

 single and double micrococci," but the slide prepared is so 

 excessively poor that nothing satisfactory can be determined 

 from it. 



From this last larva a culture was made as follows : On 

 September 1 freshly prepared strong beef broth was filtered, 

 while hot, through sterilized filter paper into a four-ounce flask 

 which had just been heated for an hour in an oven at 275-300 

 Fahrenheit. This was stopped at once with a three-inch plug 

 of raw cotton, freshly sterilized by several hours' heating as 

 above, and was boiled with the plug inserted. This flask was left 

 undisturbed until the 4th September, when it remained perfectly 

 clear. It was then boiled five minutes without removing the 

 plug and left to cool. A particle of the alimentary contents of 

 the above larva, about as large as the head of a pin, was now 

 taken up on the point of a recently heated needle. The plug 

 of the flask was removed, the infection material introduced, and 

 the flask plugged again with fresh sterilized cotton still hot 

 from the oven. A check flask was set aside at the same time. 



On the 5th September the fluid was evidently turbid 

 throughout, but especially so at the edges, and a slight film was 

 apparent upon the surface. The plug was loosened, and a 

 droplet of the fluid was obtained upon a freshly heated glass 

 rod. The mounted slide of this material was, unfortunately, 

 worthless, but, from notes made at the time, it appears that the 

 bacteria occurring were rather large " double ovals," nearly 

 all motionless, but with an occasional flagellate individual. 

 Compared with the original infection material, there was no 

 question of the identity of the two. 



On the 6th September these fluids were milky, and a film 

 had formed on the glass at the edges, where the fluid had a 



