1901] MICROSCOPICAL JOURNAL. 27 
tozoa. By inoculation of cultures of the bacillus into the 
arms of several children, a student, and himself, the 
writer was successful in producing typical vesicles. Two 
other students gave no reaction; possibly they were im- 
mune. He argues that the described bacillus is in all 
probability the specific agent in vaccinia, but with regard 
to the round and oval forms found in the corneal epithel- 
ium, he hesitates to decide whether they are really vari- 
eties of the bacillus so modified by the unfavorable site on 
which they are growing or whether they are degenera- : 
tion foci in the epithelial cells themselves. The fact that 
somewhat similar shapes are found in old cultures seem 
to give countenance to the first view. Much evidence has 
been collected to show that the protozoa of Guianieri, the 
so-called cytorrycytes variole, are characteristic and spe- 
cific and as the writer has produced identical forms by 
inoculation of cultures of this bacillus, he deduces that 
the bacillus is characteristic of small-pox lymph, and in 
all probability is the exciting factor in small-pox itself. 
Further, as the organism resembles the diphtheria bacil- 
lus, he draws a paralle] between this disease and variola 
dlintaaliy and pathologically, and finds close analogies: — 
British Medical Journal. 
METHOD OF DISTINGUISHING COLONIES OF TYPHOID Ba- 
CILLI FROM THE CoLON BactLLus.—Dr. J. A. Case (Phila- 
delphia Medical Journal; Indiana Lancet) describes a 
specially prepared culture medium recommended by 
Piorkoski. This is made by taking 100 parts of urine that 
has undergone ammoniacal fermentation, to which is ad- 
ded 0.5 parts of peptone and 3.3 parts gelatin. The whole 
is heated over a water-bath for one hour, then filtered, 
placed in test-tubes and sterilized in the usual manner, 
The sterilization is repeated for ten minutes on the fol- 
lowing day. To make the test, the stool of a patient is 
first rubbed up in a mortar, and three tubes taken, which 
