made out. Search for cells showing the nucleus in the 

 monaster, metakinetic, and diaster stages. Sketch a rest- 

 ing nucleus, and the various stages of division seen in 

 your section. 



(c) Large Animal Cells. 



The ovary of a very young chicken was hardened in a saturated 

 aqueous solution of bichloride of mercury, stained en masse in borax 

 carmine, embedded in paraffin, and sectioned. 



All paraffin sections are to be mounted in the following 

 way : Bring to the distributing table a slide on which a 

 thin layer of albumen fixative (equal parts of white of 

 egg and glycerine) has been spread. The paraffin section 

 will then be fixed to the slide. Then hold the slide over a 

 gas flame until the paraffin begins to melt, next drop a few 

 drops of turpentine on the section, and at the same time 

 rock the slide backward and forward until the paraffin has 

 been dissolved. Wipe away the excess of turpentine, add 

 a small drop of balsam, and cover with a cover glass. 



Study under low power. Observe the large round cells, 

 some of which show a relatively large nucleus. Sketch 

 several as seen under low power. 



(d) Karyokinesis in Animal Cells. 



The testes of a salamander are to be removed from the animal 

 during the month of June. They were cut into small pieces, hardened 

 in Fleming's or Hermann's solution, embedded in paraffin, very thin 

 sections cut and fixed to cover glasses, and stained either in safranin 

 and licht griin 01 Haidenhain's iron-lack-hsematoxylin and acid 

 fuchsin. After staining, the sections were dehydrated, passed through 

 oil of bergamot into xylol, from which they are mounted. Come to 

 the table with a small drop of balsam on your slide. 



Study the section first under low power, and you will 

 observe that the cells are arranged in larger and smaller 

 nests. Under high power groups of cells in mitotic cell , 

 division are often met with. All the cells in such a group 

 usually present about the same stage of division. Search 

 the section thoroughly until all the stages have been seen 

 and sketched. 



