7 



and the two covers drawn apart, next fixed in ether and alcohol, 

 stained in luematoxylin and eosin, washed and dried. Mount in 

 balsam. 



Study under high power. The red blood corpuscles 

 are oval, somewhat flattened and nucleated. When seen 

 in profile they show a slight convexity in the center. Ob- 

 serve the white corpuscles which are smaller than the red. 

 Sketch several red, and the several forms of the white met 

 with. 



('f ) Pigeon Blood Stained in Hsematoxylin and 

 Eosin. 



One of the toes of a pigeon was amputated. From the flowing 

 blood a small drop was caught on the cover glass, spread, fixed, and 

 stained in htematoxylin and eosin. Mount in balsam. 



The red cells are oval, somewhat flattened, and nucle- 

 ated. Observe the white cells. Sketch red and white cor- 

 puscles. 



(g) Amoeboid Movement in White Blood Cells of 

 a Frog. 



A small quantity of blood obtained from the heart of a frog is 

 mixed with a drop of normal salt solution and covered with a slip. 

 To prevent evaporation while examining, the cover glass is fixed by 

 means of two or three drops of melted paraffin, so placed, that after 

 congealing, one-half of the drop will rest on the slide, the other on 

 the edge of the cover; the sealing is then completed by means of a 

 camel's-hair brush which has been dipped in vaseline and drawn 

 about the edge of the cover. 



In examining this preparation be very careful not to 

 bring the end of the objective in contact with the vaseline 

 on the slide. Search the specimen until you find a white 

 blood cell of somewhat irregular shape ; place it in the 

 center of the field, and make a drawing of it as it presents 

 itself to you at this time ; at intervals of one or two min- 

 utes carefully repeat the sketching until about ten sketches 

 have been made. You will no doubt see that the cell in 

 question has changed its shape a number of times, pseu- 

 dopodia may have been thrown out and again withdrawn, 

 etc. 



