i8 



(d) ' Goblet Cells Stained in Haematoxylin and 

 Eosin. 



The large intestine of a cat was macerated in 33%" alcohol 24 to 

 48 hours. Tease a small portion of the epithelium on a cover glass, 

 dry over the flame, and stain in hgematoxylin and eosiu. Mount 

 in balsam. 



Study under high power. Some of the cells observed 

 are ordinary columnar cells; others are distended with 

 mucinogen, the nucleus being pressed down into the lower 

 part of the cell ; these are the goblet cells. Sketch several 

 as seen under high power. 



(e) Isolated Ciliated Columnar Cells Stained in 



Hsematoxylin and Eosin. 



The trachea of a cat was macerated in 33% alcohol. Tease a 

 portion of the epithelium on a cover glass, and stain in hcema- 

 toxylin and eosin. Mount in balsam. 



Study under high power. Observe that the majority 

 of the cells are columnar in shape, possessing an oval 

 nucleus. Notice particularly the clusters of fine short 

 hairs adhering to the free end of some of the columnar 

 cells. A few goblet cells may be found. Sketch as seen 

 under high power. 



(f) Cross- Section of Stratified Ciliated Columnar 



Epithelium. 



The respiratory mucous membrane of a dog's nose was hardened 

 in mercuric chloride, stained in Delafield's hsematoxylin, embedded 

 in paraffin, and sectioned. Mount in balsam. 



Study under high power. This epithelium is com- 

 posed of three layers of cells, the free cells being ciliated. 

 Sketch a portion of epithelial border. 



