54 



creosote for 15 minutes, and then into turpentine. Transfer a sec- 

 tion to the slide, remove as much of the turpentine as possible, 

 cover with a large drop of balsam,, and heat the slide over the flame for 

 several minutes. Cover with a warm cover glass, and set aside to cool. 



Study under high power. Only a few of the nerve 

 cells are stained (a brownish black), but these are usually 

 brought out very clearly. The protoplasmic processes are 

 seen branching and rebranching, and the axis cylinder pro- 

 cess may now and then be traced through several fields of 

 the microscope. This process gives off only a few small 

 branches. A few neuroglia cells may also be stained; 

 they have a small body from which many fine processes 

 radiate. Sketch nerve and neuroglia cells as seen under 

 high power. 



(d) Brain Cortex Stained after Golgi's Method. 



These sections were stained according to the above mentioned 

 method. Mount in the same way as the spinal cord section. 



Study under high power. The cortical cells have a 

 triangle-shaped body ; an axis cylinder process is given 

 off from the base, which passes down into the white sub- 

 stance; the protoplasmic processes are large, especially 

 the one given off from the apex. Sketch a number of the 

 cells as seen under high power. 



(e) Cerebellum Stained after Golgi's Method. 



The section was stained as above ; mount in hard balsam as 

 directed. 



Study first under low power, and search for Purkinje's 

 cells. These are often very beautifully stained. They 

 have an oval or pear-shaped body, from the lower end of 

 which an axis cylinder process is given off, and from th3 

 other end a large protoplasmic process which usually 

 divides into two or three branches and these divide and 

 redivide until a complicated system of branches is formed. 

 A few of the nerve cells in the granular layer and some of 

 the basket cells may also be stained. Sketch the cells 

 under high power. 



