-6 9 - 



fibrils will be seen. Fixed connective tissue cells are often 

 found on the network. Sketch under this power. 



(f ) Lymph Cells. 



Cover glasses were drawn over the freshly cut surface of a lymph 

 gland, and fixed in a solution of 3 parts ether and 1 part absolute 

 alcohol for % hour. Stain in haematoxylm for 15 minutes, wash in 

 water, counter-stain in eosin for 5 minutes, again wash in water, 

 and dry between filter paper. Mount in balsam. 



Study under high power. The greater proportion of 

 the cells seen have the shape and structure of the lympho- 

 cytes found in the blood. A few mononuclear and polynu- 

 clear cells will also be found. Sketch under high power. 



(g) Spleen. 



Small pieces from the spleen of a dog were hardened in mer- 

 curic chlorid^, stained in borax carmine, embedded in paraffin, and 

 sectioned. Fix to the slide, and mount in balsam. 



Observe the capsule composed of connective tissue and 

 non-striped muscle cells. From the capsule trabeculse 

 pass into the gland, branching and forming an anastomos- 

 ing network ; their structure is similar to that of the cap- 

 sule. Within the capsule two kinds of tissue are found, 

 the Malpighiau corpuscles and the splenic pulp. The 

 former are composed of adenoid tissue, and are usually 

 found surrounding the vessels. In the pulp a framework 

 of fine fibrils and cells is found, in the meshes of which 

 red and white blood cells are seen. Sketch a portion under 

 high power. 



(h) Cover Glass Preparation of Splenic Pulp. 



Cover glasses were drawn over the freshly cut surface of a 

 spleen, and fixed in ether-alcohol solution for 1. hour. Stain in 

 hsematoxylin and eosin as (f ) of this lesson, and mount on balsam. 



Study under high power. Many red blood cells stained 

 with the eosin, and the various forms of white blood cells, 

 will be seen. Of the white blood cells the mononuclear 

 are found in much larger proportion than in circulating 

 blood. Sketch the cells as seen under high power. 



